Protamine sulphate coated poly (lactide-co-glycolide) nanoparticles of MUC-1 peptide improved cellular uptake and cytokine release in mouse antigen presenting cells
Protamine sulphate coated poly (lactide-co-glycolide) nanoparticles of MUC-1 peptide improved cellular uptake and cytokine release in mouse antigen presenting cells
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硫酸鱼精蛋白包被的 MUC-1 肽聚丙交酯乙交酯纳米粒子改善了小鼠抗原呈递细胞的细胞摄取和细胞因子释放
DOI：
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发表时间：
2020
影响因子：
3.9
通讯作者：
J. Madan
中科院分区：
文献类型：
--
作者：
K. Jyoti;O. Katare;Anjoo Kamboj;J. Madan
摘要目的以鱼精蛋白硫酸盐(PS)、M-1-PEP-PS-P-NPs为靶向抗原提呈细胞(APC)，制备MUC-1-肽(M-1-PEP)聚丙交酯-乙交酯共聚物纳米粒，诱导细胞因子释放。方法与结果采用乳液扩散蒸发法制备了M-1-PEP-PS-P-NPs，并在一系列严格的参数下进行了体外表征。优化后的M-1-PEP-PS-P-B-NPs的平均粒径和Zeta电位分别为132.21 ± 30.71 nm和6.29 ± 0.71 mV，显著高于M-1-PEP-P-NPs的71.24 ± 17.76 nm和−43.41 ± 3.37 mV。此外，50-μg/ml M-1-PEP-PS-P-B-NPs在RAW 264.7细胞中的摄取率为82.4%(p <； 0.05)，显著高于M-1-PEP-P-NPs的63.1%。与定量结果一致的是，M-1-PEP-PS-P-B-NPs还证实了RAW 264.7细胞的高级细胞摄取(CU)，而M-1-PEP-P-NPs被认为是通过多种机制，包括吞噬和网状蛋白介导的内吞作用。结论M-1-PEP-PS-P-B-NPs必须通过吸入给药途径进行体内评价，以期在肺癌异种移植模型中达到抗肿瘤的目的。
Abstract Aim MUC-1-peptide (M-1-pep) loaded poly (lactide-co-glycolide) nanoparticles were coated with protamine sulphate (PS), M-1-pep-PS-P-NPs for targeting antigen presenting cells (APCs) to evoke cytokine release. Methods and results M-1-pep-PS-P-NPs were tailored by emulsion-diffusion evaporation method and characterised in vitro under a set of rigorous parameters. The average particle size and zeta potential of optimised M-1-pep-PS-P-B-NPs was measured to be 132.21 ± 30.71 nm and 6.29 ± 0.71 mV, significantly (p < 0.01) higher than 71.24 ± 17.76-nm and −43.41 ± 3.37 mV of M-1-pep-P-NPs. Further, 50-μg/ml concentration of M-1-pep-PS-P-B-NPs displayed 82.4% cellular uptake in RAW 264.7 cells calculated in setting of fluorescence intensity significantly (p < 0.05) elevated than 63.1% of M-1-pep-P-NPs. Consistent to quantitative results, M-1-pep-PS-P-B-NPs also confirmed advanced cellular uptake (CU) in RAW 264.7 cells in contrast to M-1-pep-P-NPs suppose to be through multiple mechanisms including phagocytosis and clathrin mediated endocytosis. Conclusion M-1-pep-PS-P-B-NPs must be evaluated in vivo through inhalation route of administration for antitumor prospective in lung cancer xenograft model.
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DOI：
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发表时间：
2008
期刊：
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影响因子：
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作者：
Maura L Gillison;Anil K. Chaturvedi;Douglas R. Lowy
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Maura L Gillison;Anil K. Chaturvedi;Douglas R. Lowy
DOI：
10.1016/b978-0-12-407697-6.00004-0
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