Protein oxidative damage at the crossroads of cellular senescence, aging, and age-related diseases.
Protein oxidative damage at the crossroads of cellular senescence, aging, and age-related diseases.
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DOI：
10.1155/2012/919832
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发表时间：
2012
影响因子：
--
通讯作者：
Friguet B
中科院分区：
文献类型：
--
作者：
Baraibar MA;Liu L;Ahmed EK;Friguet B
由氧化介导的蛋白质损伤、与晚期糖化终产物和与脂质过氧化产物形成的蛋白质加合物已经在衰老和年龄相关疾病（诸如神经变性疾病）期间被牵涉。还描述了在人成纤维细胞的复制性衰老后增加的蛋白质修饰，人成纤维细胞是用于研究体外衰老的有效模型。然而，这些修饰的蛋白质可能影响衰老表型的发展和年龄相关疾病的发病机制仍然难以捉摸。在这项研究中，我们进行了计算机模拟的方法来证明这些受损蛋白质影响的分子因子和细胞通路。通过羰基化，糖基化和脂质过氧化产物修饰的蛋白质在衰老和年龄相关疾病的数据库的建立和比较，在体外细胞复制衰老过程中确定的蛋白质。发现参与中间代谢的酶所证明的常见细胞途径被这些修饰靶向，尽管已经检查了不同的组织。这些结果强调了蛋白质修饰在衰老和年龄相关疾病中细胞代谢受损的潜在作用。
Protein damage mediated by oxidation, protein adducts formation with advanced glycated end products and with products of lipid peroxidation, has been implicated during aging and age-related diseases, such as neurodegenerative diseases. Increased protein modification has also been described upon replicative senescence of human fibroblasts, a valid model for studying aging in vitro. However, the mechanisms by which these modified proteins could impact on the development of the senescent phenotype and the pathogenesis of age-related diseases remain elusive. In this study, we performed in silico approaches to evidence molecular actors and cellular pathways affected by these damaged proteins. A database of proteins modified by carbonylation, glycation, and lipid peroxidation products during aging and age-related diseases was built and compared to those proteins identified during cellular replicative senescence in vitro. Common cellular pathways evidenced by enzymes involved in intermediate metabolism were found to be targeted by these modifications, although different tissues have been examined. These results underscore the potential effect of protein modification in the impairment of cellular metabolism during aging and age-related diseases.
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影响因子：
3.7
作者：
HAYFLICK, L;MOORHEAD, PS
通讯作者：
MOORHEAD, PS
影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.78.8.5009
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
Romani, AMP