A shell-less hen's egg test as infection model to determine the biocompatibility and antimicrobial efficacy of drugs and drug formulations against Pseudomonas aeruginosa.
A shell-less hen's egg test as infection model to determine the biocompatibility and antimicrobial efficacy of drugs and drug formulations against Pseudomonas aeruginosa.
复制标题
无壳鸡蛋测试作为感染模型，以确定药物和药物制剂对铜绿假单胞菌的生物相容性和抗菌功效
DOI：
10.1016/j.ijpharm.2020.119557
复制
发表时间：
2020
影响因子：
5.8
通讯作者：
D. Fischer
中科院分区：
文献类型：
--
作者：
P. Warncke;S. Fink;C. Wiegand;U. C. Hipler;D. Fischer
建立了一种基于无壳鸡蛋的铜绿假单胞菌感染试验，以研究药物和药物制剂对铜绿假单胞菌的抗菌效果。使用转移到培养皿中的预孵育的受精鸡蛋的测试系统在液体材料和细菌的受控局部应用以及细菌培养条件方面进行了优化。用妥布霉素、环丙沙星和美罗培南进行药敏试验，证实了体外感染模型的适用性。通过CellTiter®-Blue和微孔板激光散射比浊法测定的玻璃体内数据的相关性证明了卵细胞数据的有效性。通过MolecuLight i：X™技术可以实现感染进展和治疗效果的实时成像。此外，在概念验证有效性中，使用市售检眼镜同时确定生物相容性甚至刺激物的存在。因此，根据3R的概念，这种基于鸡蛋的感染模型可以弥合体外和体内模型之间的差距，用于评价抗生素敏感性，以减少动物试验。
A shell-less hen’s egg based infection test with Pseudomonas aeruginosa was established to investigate the antimicrobial efficacy of drugs and drug formulations close to thein vivosituation. The test system using preincubated fertilized chicken eggs transferred in petri dishes was optimized with respect to the controlled local application of liquid materials and bacteria as well as the bacterial cultivation conditions. The applicability of theex ovoinfection model was confirmed with antimicrobial susceptibility tests using tobramycin, ciprofloxacin and meropenem. The validity of theex ovodata was demonstrated by correlation within vitrodata of the CellTiter®-Blue and the microplate laser nephelometry assay. Real-time imaging of the progress of infection and the efficacy of the treatment could be realized by the MolecuLight i:X™ technique. Furthermore, in a proof-of-concept efficacy, biocompatibility and even the presence of irritants were determined side-by-side using commercial ophthalmics. In conclusion, this egg based infection model could bridge the gap betweenin vitroandin vivomodels for the evaluation of antimicrobial susceptibility to reduce animal tests according to the 3R concept.
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影响因子：
2.4
作者：
S. Bahrami;A. Rezaie;Z. Boroomand;M. Namavari;S. Ghavami
通讯作者：
S. Ghavami
药理学评论
影响因子：
--
作者：
H. Garges;K. Alexander
通讯作者：
K. Alexander
通过光谱多光子显微镜量化细菌次生代谢物的动态。
影响因子：
4
作者：
N. Sullivan;D. Tzeranis;Yun Wang;P. So;D. Newman
通讯作者：
D. Newman
影响因子：
2.9
作者：
Gow, NAR;Knox, Y;Thompson, WD
通讯作者：
Thompson, WD
DOI：
10.1007/s00417-016-3546-0
发表时间：
2017-02
期刊：
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
影响因子：
--
作者：
Pinnock A;Shivshetty N;Roy S;Rimmer S;Douglas I;MacNeil S;Garg P
通讯作者：
Garg P