Improving Schwann Cell Differentiation from Human Adipose Stem Cells with Metabolic Glycoengineering.
Improving Schwann Cell Differentiation from Human Adipose Stem Cells with Metabolic Glycoengineering.
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DOI：
10.3390/cells12081190
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发表时间：
2023-04-19
期刊：
影响因子：
6
通讯作者：
Jia, Xiaofeng
中科院分区：
文献类型：
--
作者：
Du, Jian;Wang, Zihui;Liu, Xiao;Hu, Cecilia;Yarema, Kevin J. J.;Jia, Xiaofeng
关键词：
雪旺细胞（SC）是促进周围神经再生的髓鞘形成细胞。当神经损伤形成时，SC被破坏，最终阻碍神经修复。由于SC有限且缓慢的扩张能力，治疗神经修复的困难加剧。由于脂肪干细胞（ASC）的SC分化能力，这些细胞的治疗用途正在出现在对抗周围神经损伤中，并且可以容易地大量收获。尽管ASC具有治疗潜力，但它们的转分化期通常需要两周以上。在这项研究中，我们证明了代谢糖工程（MGE）技术促进ASC分化为SC。具体而言，调节细胞表面唾液酸化的糖类似物Ac5ManNTProp（TProp）显著改善ASC分化，上调SC蛋白S100β和p75 NGFR表达，并升高神经营养因子神经生长因子β（NGFβ）和胶质细胞系衍生的神经营养因子（GDNF）。TProp处理在体外将SC转分化期从约两周显著缩短至两天，这有可能改善神经元再生并促进ASC在再生医学中的未来使用。
Schwann cells (SCs) are myelinating cells that promote peripheral nerve regeneration. When nerve lesions form, SCs are destroyed, ultimately hindering nerve repair. The difficulty in treating nerve repair is exacerbated due to SC’s limited and slow expansion capacity. Therapeutic use of adipose-derived stem cells (ASCs) is emerging in combating peripheral nerve injury due to these cells’ SC differentiation capability and can be harvested easily in large numbers. Despite ASC’s therapeutic potential, their transdifferentiation period typically takes more than two weeks. In this study, we demonstrate that metabolic glycoengineering (MGE) technology enhances ASC differentiation into SCs. Specifically, the sugar analog Ac5ManNTProp (TProp), which modulates cell surface sialylation, significantly improved ASC differentiation with upregulated SC protein S100β and p75NGFR expression and elevated the neurotrophic factors nerve growth factor beta (NGFβ) and glial cell-line-derived neurotrophic factor (GDNF). TProp treatment remarkably reduced the SC transdifferentiation period from about two weeks to two days in vitro, which has the potential to improve neuronal regeneration and facilitate future use of ASCs in regenerative medicine.
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