Effects of an EPSPS-transgenic soybean line ZUTS31 on root-associated bacterial communities during field growth.
Effects of an EPSPS-transgenic soybean line ZUTS31 on root-associated bacterial communities during field growth.
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EPSPS 转基因大豆品系 ZUTS31 对田间生长期间根部相关细菌群落的影响
DOI：
10.1371/journal.pone.0192008
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Yang YH
中科院分区：
文献类型：
--
作者：
Lu GH;Tang CY;Hua XM;Cheng J;Wang GH;Zhu YL;Zhang LY;Shou HX;Qi JL;Yang YH
近20年来，转基因作物在全球范围内的商业化种植，对土壤微生物群落产生了潜在的影响，因为许多根际和根际细菌在促进植物健康和生长方面发挥着重要作用。以往的研究表明，转基因植物对土壤微生物群落，尤其是根际细菌群落产生了不同的影响。因此，本研究比较了5-烯醇式莽草酸-3-磷酸合酶转基因大豆品系（ZUTS 31或简称Z31）及其受体品种（华春3号或简称HC 3）在营养生长期、开花期和灌浆期的大豆根相关细菌群落。通过Illumina MiSeq和实时定量PCR（qPCR）对16 S rRNA基因（16 S rDNA）V4高变区扩增子进行高通量测序。我们的研究结果显示，在3个发育阶段的根相关细菌群落的整体α多样性和根相关细菌群落的β多样性在开花期之间Z31和HC 3田间生长没有显着差异。然而，在营养生长期和灌浆期，两组根际细菌群落的β多样性存在显著差异。次世代测序和qPCR分析结果表明，从开花期到鼓粒期，根系中主要固氮细菌属的相对丰度发生明显变化，其中以慢生根瘤菌属的相对丰度变化最为明显。总之，Z31发挥暂时的影响，根际细菌群落的分类多样性在营养和种子灌浆阶段相比，在田间条件下的控制。此外，从开花期到鼓粒期，大豆的发育变化对根系中主要共生固氮菌属的数量有明显的影响。
The increased worldwide commercial cultivation of transgenic crops during the past 20 years is accompanied with potential effects on the soil microbial communities, because many rhizosphere and endosphere bacteria play important roles in promoting plant health and growth. Previous studies reported that transgenic plants exert differential effects on soil microbial communities, especially rhizobacteria. Thus, this study compared the soybean root-associated bacterial communities between a 5-enolpyruvylshikimate-3-phosphate synthase -transgenic soybean line (ZUTS31 or simply Z31) and its recipient cultivar (Huachun3 or simply HC3) at the vegetative, flowering, and seed-filling stages. High-throughput sequencing of 16S rRNA gene (16S rDNA) V4 hypervariable region amplicons via Illumina MiSeq and real-time quantitative PCR (qPCR) were performed. Our results revealed no significant differences in the overall alpha diversity of root-associated bacterial communities at the three developmental stages and in the beta diversity of root-associated bacterial communities at the flowering stage between Z31 and HC3 under field growth. However, significant differences in the beta diversity of rhizosphere bacterial communities were found at the vegetative and seed-filling stages between the two groups. Furthermore, the results of next generation sequencing and qPCR showed that the relative abundances of root-associated main nitrogen-fixing bacterial genera, especially Bradyrhizobium in the roots, evidently changed from the flowering stage to the seed-filling stage. In conclusion, Z31 exerts transitory effects on the taxonomic diversity of rhizosphere bacterial communities at the vegetative and seed-filling stages compared to the control under field conditions. In addition, soybean developmental change evidently influences the main symbiotic nitrogen-fixing bacterial genera in the roots from the flowering stage to the seed-filling stage.
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影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
影响因子：
2.4
作者：
Dunfield, KE;Germida, JJ
通讯作者：
Germida, JJ
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.4
作者：
Kozich, James J.;Westcott, Sarah L.;Schloss, Patrick D.
通讯作者：
Schloss, Patrick D.
影响因子：
11
作者：
Chaparro, Jacqueline M.;Badri, Dayakar V.;Vivanco, Jorge M.
通讯作者：
Vivanco, Jorge M.