Intraspecific variation and plasticity in mitochondrial oxygen binding affinity as a response to environmental temperature.
Intraspecific variation and plasticity in mitochondrial oxygen binding affinity as a response to environmental temperature.
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DOI：
10.1038/s41598-017-16598-6
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发表时间：
2017-11-24
影响因子：
4.6
通讯作者：
Schulte PM
中科院分区：
文献类型：
--
作者：
Chung DJ;Morrison PR;Bryant HJ;Jung E;Brauner CJ;Schulte PM
线粒体功能已被认为是限制整个生物体有氧性能和相关的缺氧和耐热极限的基础，但大多数研究都集中在最大线粒体容量的措施。在这里，我们研究了线粒体氧动力学的变化是否有助于当地的适应性和可塑性，以应对温度使用两个亚种的大西洋鳉鱼（底heteroclitus）适应一系列的温度（5，15，和33 °C）。南方亚种F.与北方亚种相比，具有上级耐热性和耐缺氧性的heteroclitus表现出较低的线粒体O2 P50（较高的O2亲和力）。适应极端温度（5或33 °C）改变了两个亚种的线粒体O2 P50，这与热适应对整个生物体热耐受极限的影响一致。我们还研究了亚种之间的差异和热适应对全血Hb O2-P50的影响，以评估氧气输送的变化是否参与这些反应。与亚种间线粒体O2-P50的明显差异相反，亚种间全血Hb-O2 P50无差异。总之，这些研究结果支持线粒体氧动力学在区分整个生物体有氧性能，从而影响物种对环境变化的反应的一般作用。
Mitochondrial function has been suggested to underlie constraints on whole-organism aerobic performance and associated hypoxia and thermal tolerance limits, but most studies have focused on measures of maximum mitochondrial capacity. Here we investigated whether variation in mitochondrial oxygen kinetics could contribute to local adaptation and plasticity in response to temperature using two subspecies of the Atlantic killifish (Fundulus heteroclitus) acclimated to a range of temperatures (5, 15, and 33 °C). The southern subspecies of F. heteroclitus, which has superior thermal and hypoxia tolerances compared to the northern subspecies, exhibited lower mitochondrial O2 P50 (higher O2 affinity). Acclimation to thermal extremes (5 or 33 °C) altered mitochondrial O2 P50 in both subspecies consistent with the effects of thermal acclimation on whole-organism thermal tolerance limits. We also examined differences between subspecies and thermal acclimation effects on whole-blood Hb O2-P50 to assess whether variation in oxygen delivery is involved in these responses. In contrast to the clear differences between subspecies in mitochondrial O2-P50 there were no differences in whole-blood Hb-O2 P50 between subspecies. Taken together these findings support a general role for mitochondrial oxygen kinetics in differentiating whole-organism aerobic performance and thus in influencing species responses to environmental change.
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影响因子：
5
作者：
Kim, Min-Ji;Kim, Bong-Gyu;Ahn, Joong-Hoon
通讯作者：
Ahn, Joong-Hoon
DOI：
10.1073/pnas.1211001109
发表时间：
2012-10-02
影响因子：
11.1
作者：
Qualley, Anthony V.;Widhalm, Joshua R.;Dudareva, Natalia
通讯作者：
Dudareva, Natalia
影响因子：
2.9
作者：
Cifani, Carlo;Di B, Maria Vittoria Micioni;Massi, Maurizio
通讯作者：
Massi, Maurizio
影响因子：
4.6
作者：
Bai Y;Bi H;Zhuang Y;Liu C;Cai T;Liu X;Zhang X;Liu T;Ma Y
通讯作者：
Ma Y
影响因子：
2.9
作者：
Jones, J. Andrew;Collins, Shannon M.;Koffas, Mattheos A. G.
通讯作者：
Koffas, Mattheos A. G.