Identification of the plasticity-relevant fucose-alpha(1-2)-galactose proteome from the mouse olfactory bulb.
Identification of the plasticity-relevant fucose-alpha(1-2)-galactose proteome from the mouse olfactory bulb.
复制标题
DOI：
10.1021/bi900640x
复制
发表时间：
2009-08-04
期刊：
影响因子：
2.9
通讯作者：
Hsieh-Wilson, Linda C.
中科院分区：
文献类型：
--
作者：
Murrey, Heather E.;Ficarro, Scott B.;Krishnamurthy, Chithra;Domino, Steven E.;Peters, Eric C.;Hsieh-Wilson, Linda C.
Fucose-α(1−2)-galactose [Fucα(1−2)Gal] sugars have been implicated in the molecular mechanisms that underlie neuronal development, learning, and memory. However, an understanding of their precise roles has been hampered by a lack of information regarding Fucα(1−2)Gal glycoproteins. Here, we report the first proteomic studies of this plasticity-relevant epitope. We identify five classes of putative Fucα(1−2)Gal glycoproteins: cell adhesion molecules, ion channels and solute carriers/transporters, ATP-binding proteins, synaptic vesicle-associated proteins, and mitochondrial proteins. In addition, we show that Fucα(1−2)Gal glycoproteins are enriched in the developing mouse olfactory bulb (OB) and exhibit a distinct spatiotemporal expression that is consistent with the presence of a “glycocode” to help direct olfactory sensory neuron (OSN) axonal pathfinding. We find that expression of Fucα(1−2)Gal sugars in the OB is regulated by the α(1−2)fucosyltransferase FUT1. FUT1-deficient mice exhibit developmental defects, including fewer and smaller glomeruli and a thinner olfactory nerve layer, suggesting that fucosylation contributes to OB development. Our findings significantly expand the number of Fucα(1−2)Gal glycoproteins and provide new insights into the molecular mechanisms by which fucosyl sugars contribute to neuronal processes.
登录
查看更多内容
影响因子：
64.5
作者：
Mombaerts, P;Wang, F;Axel, R
通讯作者：
Axel, R
影响因子：
2.9
作者：
KRUG, M;JORK, R;MATTHIES, H
通讯作者：
MATTHIES, H
影响因子：
5.3
作者：
Cho, Jin Hyung;Lepine, Manon;Cloutier, Jean-Francois
通讯作者：
Cloutier, Jean-Francois
影响因子：
7
作者：
Bai, Yu;Markham, Kelly;Schmitt-Ulms, Gerold
通讯作者：
Schmitt-Ulms, Gerold
影响因子：
15
作者：
Kalovidouris, SA;Gama, CI;Hsieh-Wilson, LC
通讯作者：
Hsieh-Wilson, LC