Glucocorticoid-regulated localization of cell surface glycoproteins in rat hepatoma cells is mediated within the Golgi complex.
Glucocorticoid-regulated localization of cell surface glycoproteins in rat hepatoma cells is mediated within the Golgi complex.
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DOI：
10.1083/jcb.106.5.1463
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发表时间：
1988-05
期刊：
影响因子：
--
通讯作者：
Firestone GL
中科院分区：
文献类型：
--
作者：
Haffar OK;Aponte GW;Bravo DA;John NJ;Hess RT;Firestone GL
糖皮质激素调节稳定感染的大鼠肝癌细胞系M1.54细胞中细胞表面和细胞外小鼠乳腺肿瘤病毒（MMTV）糖蛋白的翻译后成熟和分选。暴露于莫能菌素显著降低了病毒糖蛋白的蛋白水解成熟和外化，导致单个70，000-Mr糖基化多聚蛋白（指定为gp 70）的稳定细胞积累。莫能菌素处理的细胞的细胞表面和细胞内特异性免疫沉淀显示，gp 70可以定位到细胞表面，只有在1 μ M地塞米松的存在下，而在未诱导的细胞gp 70是不可逆的隔离在细胞内的隔间。寡糖加工动力学的分析表明，gp 70获得的耐糖苷内切酶H与65分钟，在激素的存在或不存在下的半衰期。与此相反，gp 70是无效的半乳糖基化后，60分钟的滞后在未诱导的细胞，而迅速获得这种糖修饰的地塞米松的存在下。此外，在莫能菌素存在或不存在的情况下，MMTV糖蛋白未能被半乳糖基化在糖皮质激素诱导的CR 4细胞，补体选择的分选变异缺陷的糖皮质激素调节区室化的病毒糖蛋白的细胞表面。由于地塞米松对细胞器形态或总细胞表面半乳糖基化物质的产生没有明显的全局影响，我们得出结论，糖皮质激素通过调节高尔基体内的高选择性步骤诱导细胞表面MMTV糖蛋白的定位，该步骤在获得糖苷内切酶H-抗性寡糖侧链后，但在半乳糖附着之前或在半乳糖附着位点。
Glucocorticoid hormones regulate the post-translational maturation and sorting of cell surface and extracellular mouse mammary tumor virus (MMTV) glycoproteins in M1.54 cells, a stably infected rat hepatoma cell line. Exposure to monensin significantly reduced the proteolytic maturation and externalization of viral glycoproteins resulting in a stable cellular accumulation of a single 70,000-Mr glycosylated polyprotein (designated gp70). Cell surface- and intracellular-specific immunoprecipitations of monensin-treated cells revealed that gp70 can be localized to the cell surface only in the presence of 1 microM dexamethasone, while in uninduced cells gp70 is irreversibly sequestered in an intracellular compartment. Analysis of oligosaccharide processing kinetics demonstrated that gp70 acquired resistance to endoglycosidase H with a half-time of 65 min in the presence or absence of hormone. In contrast, gp70 was inefficiently galactosylated after a 60-min lag in uninduced cells while rapidly acquiring this carbohydrate modification in the presence of dexamethasone. Furthermore, in the absence or presence of monensin, MMTV glycoproteins failed to be galactosylated in hormone-induced CR4 cells, a complement-selected sorting variant defective in the glucocorticoid-regulated compartmentalization of viral glycoproteins to the cell surface. Since dexamethasone had no apparent global effects on organelle morphology or production of total cell surface-galactosylated species, we conclude that glucocorticoids induce the localization of cell surface MMTV glycoproteins by regulating a highly selective step within the Golgi apparatus after the acquisition of endoglycosidase H- resistant oligosaccharide side chains but before or at the site of galactose attachment.
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影响因子：
7.8
作者：
Hendershot, L;Bole, D;Kohler, G;Kearney, J F
通讯作者：
Kearney, J F
DOI：
10.1073/pnas.78.12.7453
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DUNPHY, WG;FRIES, E;ROTHMAN, JE
通讯作者：
ROTHMAN, JE
影响因子：
3.7
作者：
BOTTENSTEIN, JE;SATO, GH
通讯作者：
SATO, GH
DOI：
10.1083/jcb.103.6.2323
发表时间：
1986-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Firestone GL;John NJ;Yamamoto KR
通讯作者：
Yamamoto KR
影响因子：
4
作者：
FIRESTONE, GL;JOHN, NJ;COOK, PW
通讯作者：
COOK, PW