5-methyl-cytosine and 5-hydroxy-methyl-cytosine in the genome of Biomphalaria glabrata, a snail intermediate host of Schistosoma mansoni.
5-methyl-cytosine and 5-hydroxy-methyl-cytosine in the genome of Biomphalaria glabrata, a snail intermediate host of Schistosoma mansoni.
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DOI：
10.1186/1756-3305-6-167
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发表时间：
2013-06-06
影响因子：
3.2
通讯作者：
Cosseau C
中科院分区：
文献类型：
--
作者：
Fneich S;Dheilly N;Adema C;Rognon A;Reichelt M;Bulla J;Grunau C;Cosseau C
光滑双脐螺是曼氏血吸虫（Schistosoma mansoni）的中间宿主，曼氏血吸虫是一种引起人类肠道血吸虫病的二殖吸虫寄生虫。据估计，74个国家有2亿人患有血吸虫病，就发病率而言，这是继疟疾之后最严重的热带疾病。表观遗传学信息告知基因活性的状态是可遗传的，其变化是可逆的，并且不基于DNA序列。表观遗传机制产生的变异性为潜在的可遗传表型变异提供了来源，因此可能参与对环境约束的适应。表型变异在宿主-寄生虫相互作用中特别重要，其中选择压力和进化速率都很高。在这种情况下，表观遗传变化预计是表型可塑性和宿主与寄生虫之间的共适应的主要驱动力。因此，随着寄生虫载体或中间宿主无脊椎动物基因组的表征，了解表观遗传机制如何发挥作用以更好地破译宿主和寄生虫之间的相互作用也是至关重要的。CpGo/e比率用作研究B中CpG甲基化的发生的代表。glabrata编码区。B中DNA甲基化的存在。glabrata也通过几种实验方法证实：用异构酶的限制酶消化、基于亚硫酸氢盐转化的技术和LC-MS/MS分析。在这项工作中，我们报告，DNA甲基化，这是一个载体的表观遗传信息，发生在B。glabrata;大约2%的胞嘧啶核苷酸被甲基化。我们描述了B的甲基化机制。光滑的甲基化主要发生在CpG位点，在与管家功能相关的基因的编码区中以高比例存在。我们还证明了亚硫酸氢盐处理甲基化发生在多个拷贝的光轮，转座因子。本研究第一次详细介绍了DNA甲基化，它是B中表观遗传信息的载体之一。光滑的我们在B中观察到的DNA甲基化的一般特征。glabrata基因组符合表观遗传学研究报告的其他无脊椎动物物种。
Biomphalaria glabrata is the mollusc intermediate host for Schistosoma mansoni, a digenean flatworm parasite that causes human intestinal schistosomiasis. An estimated 200 million people in 74 countries suffer from schistosomiasis, in terms of morbidity this is the most severe tropical disease after malaria. Epigenetic information informs on the status of gene activity that is heritable, for which changes are reversible and that is not based on the DNA sequence. Epigenetic mechanisms generate variability that provides a source for potentially heritable phenotypic variation and therefore could be involved in the adaptation to environmental constraint. Phenotypic variations are particularly important in host-parasite interactions in which both selective pressure and rate of evolution are high. In this context, epigenetic changes are expected to be major drivers of phenotypic plasticity and co-adaptation between host and parasite. Consequently, with characterization of the genomes of invertebrates that are parasite vectors or intermediate hosts, it is also essential to understand how the epigenetic machinery functions to better decipher the interplay between host and parasite. The CpGo/e ratios were used as a proxy to investigate the occurrence of CpG methylation in B. glabrata coding regions. The presence of DNA methylation in B. glabrata was also confirmed by several experimental approaches: restriction enzymatic digestion with isoschizomers, bisulfite conversion based techniques and LC-MS/MS analysis. In this work, we report that DNA methylation, which is one of the carriers of epigenetic information, occurs in B. glabrata; approximately 2% of cytosine nucleotides are methylated. We describe the methylation machinery of B. glabrata. Methylation occurs predominantly at CpG sites, present at high ratios in coding regions of genes associated with housekeeping functions. We also demonstrate by bisulfite treatment that methylation occurs in multiple copies of Nimbus, a transposable element. This study details DNA methylation for the first time, one of the carriers of epigenetic information in B. glabrata. The general characteristics of DNA methylation that we observed in the B. glabrata genome conform to what epigenetic studies have reported from other invertebrate species.
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影响因子：
3.7
作者：
Krauss V;Eisenhardt C;Unger T
通讯作者：
Unger T
影响因子：
2.6
作者：
Glastad, K. M.;Hunt, B. G.;Goodisman, M. A. D.
通讯作者：
Goodisman, M. A. D.
影响因子：
64.8
作者：
COULONDRE, C;MILLER, JH;GILBERT, W
通讯作者：
GILBERT, W
影响因子：
56.9
作者：
Goll, MG;Kirpekar, F;Bestor, TH
通讯作者：
Bestor, TH
影响因子：
3.7
作者：
Deleury E;Dubreuil G;Elangovan N;Wajnberg E;Reichhart JM;Gourbal B;Duval D;Baron OL;Gouzy J;Coustau C
通讯作者：
Coustau C