Specific versus non-specific immune responses in an invertebrate species evidenced by a comparative de novo sequencing study.
Specific versus non-specific immune responses in an invertebrate species evidenced by a comparative de novo sequencing study.
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DOI：
10.1371/journal.pone.0032512
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Coustau C
中科院分区：
文献类型：
--
作者：
Deleury E;Dubreuil G;Elangovan N;Wajnberg E;Reichhart JM;Gourbal B;Duval D;Baron OL;Gouzy J;Coustau C
我们目前对无脊椎动物先天免疫的功能和进化历史的了解主要来自于对一些属于外生动物的模式物种的研究。特别是，黑胃果蝇对真菌和革兰氏阳性或革兰氏阴性细菌特异性反应的信号通路的表征挑战了我们对无脊椎动物非特异性免疫的原始观点。然而，光藻动物物种中仍有许多有待阐明。为了研究淡水蜗牛Biomphalaria glabrata免疫应答的全局特异性，我们使用大量Illumina测序5 '端cdna来比较革兰氏阳性或革兰氏阴性细菌或酵母攻击后的表达谱。文库的5′端cDNA测序获得了超过1200万个高质量的reads。为了将这些短序列与表达基因连接起来，我们通过自动组装和注释公共数据库中提供的758,510个冗余序列（est, mrna），建立了一个参考转录组数据库。对Illumina reads进行计算分析，然后进行多变量分析，鉴定出免疫攻击后差异表达的1685个候选转录本，显示攻击特异性表达的转录本与低特异性或非特异性差异表达的转录本之间的比例为两倍。对于随机选择的候选子集，使用定量PCR验证了差异表达。注释候选者的预测函数(约为。700个不相同的序列)属于很大程度上延伸到相似的功能类别或蛋白质类型。这项工作在先前的基因发现和表达研究的基础上进行了显著扩展，并表明对各种病原体的反应可能涉及相似的免疫过程或信号通路，但属于多基因家族的不同基因。这些结果提出了在特定无脊椎动物免疫反应的进化中基因复制和获得类似功能多样性的重要性的问题。
Our present understanding of the functioning and evolutionary history of invertebrate innate immunity derives mostly from studies on a few model species belonging to ecdysozoa. In particular, the characterization of signaling pathways dedicated to specific responses towards fungi and Gram-positive or Gram-negative bacteria in Drosophila melanogaster challenged our original view of a non-specific immunity in invertebrates. However, much remains to be elucidated from lophotrochozoan species. To investigate the global specificity of the immune response in the fresh-water snail Biomphalaria glabrata, we used massive Illumina sequencing of 5′-end cDNAs to compare expression profiles after challenge by Gram-positive or Gram-negative bacteria or after a yeast challenge. 5′-end cDNA sequencing of the libraries yielded over 12 millions high quality reads. To link these short reads to expressed genes, we prepared a reference transcriptomic database through automatic assembly and annotation of the 758,510 redundant sequences (ESTs, mRNAs) of B. glabrata available in public databases. Computational analysis of Illumina reads followed by multivariate analyses allowed identification of 1685 candidate transcripts differentially expressed after an immune challenge, with a two fold ratio between transcripts showing a challenge-specific expression versus a lower or non-specific differential expression. Differential expression has been validated using quantitative PCR for a subset of randomly selected candidates. Predicted functions of annotated candidates (approx. 700 unisequences) belonged to a large extend to similar functional categories or protein types. This work significantly expands upon previous gene discovery and expression studies on B. glabrata and suggests that responses to various pathogens may involve similar immune processes or signaling pathways but different genes belonging to multigenic families. These results raise the question of the importance of gene duplication and acquisition of paralog functional diversity in the evolution of specific invertebrate immune responses.
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影响因子：
12.3
作者：
Gerardo NM;Altincicek B;Anselme C;Atamian H;Barribeau SM;de Vos M;Duncan EJ;Evans JD;Gabaldón T;Ghanim M;Heddi A;Kaloshian I;Latorre A;Moya A;Nakabachi A;Parker BJ;Pérez-Brocal V;Pignatelli M;Rahbé Y;Ramsey JS;Spragg CJ;Tamames J;Tamarit D;Tamborindeguy C;Vincent-Monegat C;Vilcinskas A
通讯作者：
Vilcinskas A
影响因子：
4.7
作者：
De Zoysa, Mahanama;Whang, Ilson;Lee, Jehee
通讯作者：
Lee, Jehee
通过数字基因表达分析，成功免疫反应在牡蛎crassostrea gigas中成功免疫反应的整体转录组分析。
影响因子：
3.7
作者：
de Lorgeril J;Zenagui R;Rosa RD;Piquemal D;Bachère E
通讯作者：
Bachère E
DOI：
10.1073/pnas.0704967104
发表时间：
2007-07-10
影响因子：
11.1
作者：
Baxter, Richard H. G.;Chang, Chung-I;Deisenhofer, Johann
通讯作者：
Deisenhofer, Johann
影响因子：
2.4
作者：
Coustau, C;Mitta, G;Modat, S
通讯作者：
Modat, S