Inhalation of carbon monoxide reduces skeletal muscle injury after hind limb ischemia-reperfusion injury in mice.
Inhalation of carbon monoxide reduces skeletal muscle injury after hind limb ischemia-reperfusion injury in mice.
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DOI：
10.1016/j.amjsurg.2011.05.005
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发表时间：
2012-04
影响因子：
3
通讯作者：
Watkins, Michael T.
中科院分区：
文献类型：
--
作者：
Patel, Rajendra;Albadawi, Hassan;Steudel, Wolfgang;Hashmi, Faraz F.;Kang, Jeanwan;Yoo, Hyung-Jin;Watkins, Michael T.
本研究旨在探讨吸入一氧化碳(CO)能否改善肢体缺血再灌流(IR)后骨骼肌损伤，调节内源性HO-1的表达，改善组织完整性和炎症指标。C57BL6小鼠吸入CO(250ppm)或室内空气，缺血1.5小时后肢体再灌注3或6小时(总治疗时间4.5或7.5小时)。在再灌流初期，所有小鼠都只呼吸室内空气，直到缺血发作后24小时。在CO治疗结束或再灌流24小时时处死小鼠。对骨骼肌进行组织学和生化分析。联合治疗7.5小时可保护骨骼肌免受骨骼肌损伤的组织学和结构证据的影响。一氧化碳染毒7.5小时后，小鼠血清和组织细胞因子显著降低(p<；0.05)。一氧化碳处理组小鼠的微管蛋白、血红素加氧酶和三磷酸腺苷水平较高。吸入CO对IR后的肌肉结构损伤和能量耗竭具有保护作用。
The purpose of this study was to determine if inhaled carbon monoxide (CO) can ameliorate skeletal muscle injury, modulate endogenous heme oxygenase-1 (HO) expression, improve indices of tissue integrity and inflammation following hind limb ischemia reperfusion(IR). C57BL6 mice inhaling CO (250ppm) or room air were subjected to 1.5 hrs of ischemia followed by limb reperfusion for either 3 or 6 hours (total treatment time of 4.5 or 7.5 hrs). After the initial period of reperfusion, all mice breathed only room air until 24 hours after the onset of ischemia. Mice were sacrificed at either the end of CO treatment or at 24 hours reperfusion. Skeletal muscle was subjected to histologic and biochemical analysis. CO treatment for 7.5 hours protected skeletal muscle from histologic and structural evidence of skeletal muscle injury. Serum and tissue cytokines were significantly reduced (p<0.05) in mice treated with CO for 7.5 hours. Tubulin, Heme Oxygenase, and ATP levels were higher in CO treated mice. Inhaled CO protected muscle from structural injury and energy depletion following IR.
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DOI：
10.1161/01.atv.0000118279.74056.8a
发表时间：
2004-03-01
影响因子：
8.7
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1152/ajpgi.00144.2007
发表时间：
2008-01-01
影响因子：
4.5
作者：
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影响因子：
6.1
作者：
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