The drnf1 Gene from the Drought-Adapted Cyanobacterium Nostoc flagelliforme Improved Salt Tolerance in Transgenic Synechocystis and Arabidopsis Plant.
The drnf1 Gene from the Drought-Adapted Cyanobacterium Nostoc flagelliforme Improved Salt Tolerance in Transgenic Synechocystis and Arabidopsis Plant.
复制标题
来自适应干旱的发菜蓝藻的 drnf1 基因提高了转基因集胞藻和拟南芥植物的耐盐性
作者：
Cui L;Liu Y;Yang Y;Ye S;Luo H;Qiu B;Gao X
环境非生物胁迫是包括土壤植物在内的耐受性较差的生物的限制因素。原核生物非生物胁迫耐受相关基因具有良好的转基因应用前景。发菜是一种耐旱的原核生物资源，可用于基因挖掘。本研究对N.鞭毛状藻，其含有P-环NTR（核苷-三磷酸酶）结构域，通过在两种模式生物集胞藻属PCC 6803和拟南芥中异源表达。发现DRNF 1可以赋予两种转基因生物显著的耐盐性。在盐胁迫下，转DRNF 1基因集胞藻与野生型集胞藻相比，能够提高呼吸速率，减缓胞外多糖的积累，上调与葡萄糖甘油合成、Na+/H+反向转运和糖代谢相关的耐盐相关基因的表达，维持更好的K+/Na+稳态。这些结果表明DRNF 1可能通过影响呼吸代谢和间接调节重要耐盐基因的表达来促进植物的耐盐性。拟南芥被用来评估DRNF 1在植物中的耐盐性赋予潜力。结果表明，在盐胁迫条件下，它能促进转基因植物种子的萌发和幼苗的生长。总之，一个新的原核耐盐基因从N。本研究鉴定并鉴定了鞭毛状藻基因，丰富了植物基因工程的候选基因库。
Environmental abiotic stresses are limiting factors for less tolerant organisms, including soil plants. Abiotic stress tolerance-associated genes from prokaryotic organisms are supposed to have a bright prospect for transgenic application. The drought-adapted cyanobacterium Nostoc flagelliforme is arising as a valuable prokaryotic biotic resource for gene excavation. In this study, we evaluated the salt-tolerant function and application potential of a candidate gene drnf1 from N. flagelliforme, which contains a P-loop NTPase (nucleoside-triphosphatase) domain, through heterologous expression in two model organisms Synechocystis sp. PCC 6803 and Arabidopsis thaliana. It was found that DRNF1 could confer significant salt tolerance in both transgenic organisms. In salt-stressed transgenic Synechocystis, DRNF1 could enhance the respiration rate; slow-down the accumulation of exopolysaccharides; up-regulate the expression of salt tolerance-related genes at a higher level, such as those related to glucosylglycerol synthesis, Na+/H+ antiport, and sugar metabolism; and maintain a better K+/Na+ homeostasis, as compared to the wild-type strain. These results imply that DRNF1 could facilitate salt tolerance by affecting the respiration metabolism and indirectly regulating the expression of important salt-tolerant genes. Arabidopsis was employed to evaluate the salt tolerance-conferring potential of DRNF1 in plants. The results show that it could enhance the seed germination and shoot growth of transgenic plants under saline conditions. In general, a novel prokaryotic salt-tolerant gene from N. flagelliforme was identified and characterized in this study, enriching the candidate gene pool for genetic engineering in plants.
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