Genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China.
Genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China.
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DOI：
10.1186/s13099-017-0209-6
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发表时间：
2017
期刊：
影响因子：
4.2
通讯作者：
Shao H
中科院分区：
文献类型：
--
作者：
Zhang T;Dong J;Cheng Y;Lu Q;Luo Q;Wen G;Liu G;Shao H
弯曲杆菌被认为是人类细菌性肠胃炎的主要原因，其中家禽是主要宿主。弯曲杆菌污染的鸡肉产品是人类感染弯曲杆菌的主要原因。本研究调查了弯曲杆菌（Campylobacter）在华中地区鸡群中的流行情况，并对其基因型多样性、耐药性和生物膜特征进行了分析。从中部地区养鸡场和活禽市场共分离出206株弯曲杆菌，其中空肠弯曲杆菌166株，大肠弯曲杆菌40株。多位点序列分型和系统发育分析表明，弯曲杆菌分离株具有多样化的遗传背景，覆盖了国内报道的大多数优势克隆复合体（CCs）。最常见的是CC-464、CC-1150、CC-353和CC-828。所有分离株均对诺氟沙星、环丙沙星和头孢唑林耐药，对氟喹诺酮类药物、β-内酰胺类药物和四环素类药物普遍耐药。133株菌株具有形成生物膜的能力，其中以CC-21、CC-48、CC-677和CC-45为主要遗传分支的菌株形成生物膜的能力显著低于其他遗传分支（p < 0.05）。然而，总的来说，形成生物膜的能力在不同的遗传分支之间存在差异，这表明生物膜的形成具有复杂的遗传背景，而不仅仅是遗传谱系。与不能形成生物膜的菌株相比，产生生物膜的菌株对氨苄西林、新霉素、磺胺甲恶唑、阿米卡星、克林霉素和红霉素的耐药性显著提高（p < 0.05）。据我们所知，本文首次报道了华中地区鸡源弯曲杆菌的基因型多样性、耐药性和生物膜形成能力之间的关系，显示了生物膜在耐药性中的潜在重要性。这项研究将有助于我们更好地了解弯曲杆菌的流行病学和耐药性。本文的在线版本（10.1186/s13099-017-0209-6）包含补充内容，仅供授权用户使用。
Campylobacter is considered to be the leading cause of human bacterial gastroenteritis, of which poultry is the main reservoir. Campylobacter contaminated chicken products are a major cause of human Campylobacter infection. In this study, the prevalence of Campylobacter in chicken in central China was investigated, and the genotypic diversity, antimicrobial resistance and biofilm of these isolates were characterized. A total of 206 Campylobacter isolates, including 166 C. jejuni and 40 C. coli, were isolated from chicken farms and live poultry markets in central China. Multilocus sequence typing and phylogenetic analysis showed that the Campylobacter isolates had diverse genetic backgrounds, which covered most of the dominant clone complexes (CCs) reported throughout China. The most prevalent CCs were CC-464, CC-1150, CC-353, and CC-828. All the isolates showed resistance to norfloxacin, ciprofloxacin and Cefazolin, and a prevalent resistance to fluoroquinolones, β-lactams and tetracyclines was also observed. Among all the isolates, 133 strains showed the ability to form biofilm, thereinto, the isolates in two genetic branches, mainly including CC-21, CC-48, CC-677 and CC-45, showed a significantly lower ability to form biofilm than other genetic branches (p < 0.05). However, in general, the ability to form biofilm varied among different genetic branches, suggesting a complex genetic background to biofilm formation, but not only the genetic lineages. Compared with the strains unable to form biofilm, biofilm-producing strains possessed a significantly higher resistance to ampicillin, neomycin, sulfamethoxazole, amikacin, clindamycin and erythromycin (p < 0.05). To the best of our knowledge, this is the first report on the relationship of the genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China, which showed the potential importance of biofilm in antimicrobial resistance. This study will help us better understand the epidemiology and antimicrobial resistance of Campylobacter. The online version of this article (10.1186/s13099-017-0209-6) contains supplementary material, which is available to authorized users.
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