Synthesis and evaluation of self-calibrating ratiometric viscosity sensors.
Synthesis and evaluation of self-calibrating ratiometric viscosity sensors.
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DOI：
10.1039/c0ob01042a
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发表时间：
2011-05-07
影响因子：
3.2
通讯作者：
Theodorakis EA
中科院分区：
文献类型：
--
作者：
Yoon HJ;Dakanali M;Lichlyter D;Chang WM;Nguyen KA;Nipper ME;Haidekker MA;Theodorakis EA
我们描述了一个家庭的自校准染料，提供流体粘度的比率测量的设计，合成和荧光配置文件。该设计是基于共价连接的主要荧光团（参考），显示粘度无关的荧光发射与次要荧光团（传感器），表现出粘度敏感的荧光发射。荧光性质的表征是通过单元的单独激发和通过从参比染料到传感染料的共振能量转移进行的。为了优化粘度测量的整体亮度和灵敏度，对荧光团和连接体长度的化学结构进行了评价。我们还介绍了此类比率染料在脂质体模型中检测膜粘度变化的应用。
We describe the design, synthesis and fluorescent profile of a family of self-calibrating dyes that provide ratiometric measurements of fluid viscosity. The design is based on covalently linking a primary fluorophore (reference) that displays a viscosity-independent fluorescence emission with a secondary fluorophore (sensor) that exhibits a viscosity-sensitive fluorescence emission. Characterization of fluorescent properties was made with separate excitation of the units and through Resonance Energy Transfer from the reference to the sensor dye. The chemical structures of both fluorophores and the linker length have been evaluated in order to optimize the overall brightness and sensitivity of the viscosity measurements. We also present an application of such ratiometric dyes for the detection of membrane viscosity changes in a liposome model.
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影响因子：
5.6
作者：
Haidekker MA;Theodorakis EA
通讯作者：
Theodorakis EA
影响因子：
3.4
作者：
Goodwin, JS;Drake, KR;Kenworthy, AK
通讯作者：
Kenworthy, AK
影响因子：
3.2
作者：
Haidekker, Mark A.;Theodorakis, Emmanuel A.
通讯作者：
Theodorakis, Emmanuel A.
DOI：
10.1152/ajpheart.00712.2001
发表时间：
2002-05-01
影响因子：
4.8
作者：
Haidekker, MA;Tsai, AG;Intaglietta, M
通讯作者：
Intaglietta, M
影响因子：
2.1
作者：
Berthelot, T;Talbot, JC;Latxague, L
通讯作者：
Latxague, L