Ectopic expression of foxtail millet zip-like gene, SiPf40, in transgenic rice plants causes a pleiotropic phenotype affecting tillering, vascular distribution and root development
Ectopic expression of foxtail millet zip-like gene, SiPf40, in transgenic rice plants causes a pleiotropic phenotype affecting tillering, vascular distribution and root development
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转基因水稻中谷子 zip 样基因 SiPf40 的异位表达引起多效性表型，影响分蘖、维管分布和根系发育
DOI：
10.1007/s11427-010-4090-5
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发表时间：
2010-12
影响因子：
9.1
通讯作者：
Yu JingJuan
中科院分区：
文献类型：
--
作者：
Luan YunXia;Wang BaoSheng;Zhao Qian;Ao GuangMing;Yu JingJuan
植株构型决定着水稻的籽粒产量，并受分蘖、株高和穗形态等重要农艺性状的影响。许多控制腋芽的发生和生长、茎的伸长和花序结构的关键基因已经被分离和分析。以前的研究表明，SiPf 40，这是确定从谷子（Setaria italica）未成熟种子cDNA文库，导致额外的分支和分蘖SiPf 40转基因烟草和谷子，分别。为了再次确认其功能，我们产生了在泛素启动子控制下过表达SiPf 40的转基因水稻植株。SiPf 40过表达的转基因植物比野生型植物具有更大的分蘖数和更宽的分蘖角。它们的根构型通过促进侧根发育而改变，木质部和韧皮部在维管束中的分布也受到影响。激素水平分析表明，与野生型相比，转SiPf 40基因植株的吲哚乙酸/玉米素（IAA/ZR）和吲哚乙酸/赤霉素（IAA/GA）的比值降低。这些发现有力地表明SiPf 40在植物结构中起着重要作用。
Plant architecture determines grain production in rice (Oryza sativa) and is affected by important agronomic traits such as tillering, plant height, and panicle morphology. Many key genes involved in controlling the initiation and outgrowth of axillary buds, the elongation of stems, and the architecture of inflorescences have been isolated and analyzed. Previous studies have shown that SiPf40, which was identified from a foxtail millet (Setaria italica) immature seed cDNA library, causes extra branches and tillers in SiPf40-transgenic tobacco and foxtail millet, respectively. To reconfirm its function, we generated transgenic rice plants overexpressing SiPf40 under the control of the ubiquitin promoter. SiPf40-overexpressing transgenic plants have a greater tillering number and a wider tiller angle than wild-type plants. Their root architecture is modified by the promotion of lateral root development, and the distribution of xylem and phloem in the vascular bundle is affected. Analysis of hormone levels showed that the ratios of indole-3-acetic acid/zeatin (IAA/ZR) and IAA/gibberellic acid (IAA/GA) decreased in SiPf40-transgenic plants compared with wild-type plants. These findings strongly suggest that SiPf40 plays an important role in plant architecture.
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影响因子：
11.6
作者：
Zhao, Yu;Hu, Yongfeng;Zhou, Dao-Xiu
通讯作者：
Zhou, Dao-Xiu
ZmZLP1（编码玉米内质网定位锌转运蛋白的基因）的克隆和表征
影响因子：
3
作者：
Wang, Bao-Sheng;Ao, Guang-Ming;Yu, Jing-Juan;Xu, Yao-Guang;Zhao, Qian
通讯作者：
Zhao, Qian
影响因子：
7.2
作者：
Liu, HJ;Wang, SF;Wu, P
通讯作者：
Wu, P
影响因子：
4.1
作者：
Monna, L;Kitazawa, N;Minobe, Y
通讯作者：
Minobe, Y
影响因子：
7.4
作者：
Ramesh, SA;Shin, R;Schachtman, DP
通讯作者：
Schachtman, DP