Cloning and characterisation of ZmZLP1, a gene encoding an endoplasmic reticulum-localised zinc transporter in Zea mays
Cloning and characterisation of ZmZLP1, a gene encoding an endoplasmic reticulum-localised zinc transporter in Zea mays
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ZmZLP1（编码玉米内质网定位锌转运蛋白的基因）的克隆和表征
DOI：
10.1071/fp09045
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发表时间：
2010-03
影响因子：
3
通讯作者：
Zhao, Qian
中科院分区：
文献类型：
--
作者：
Wang, Bao-Sheng;Ao, Guang-Ming;Yu, Jing-Juan;Xu, Yao-Guang;Zhao, Qian
ZmZLP 1（ZmZIP-like protein）基因是从玉米（Zea mays L.）（玉米）花粉。生物信息学分析表明，ZmZLP 1与金属离子转运蛋白ZIP（ZRT/IRT样蛋白）家族具有许多共同的特征。在一般的营养条件下，ZmZLP 1的表达在成熟的花粉和，不太强烈，在雄花序中检测到，而诱导ZmZLP 1转录观察到在根锌剥夺12小时后。GFP的可视化显示ZmZLP 1靶向内质网（ER）。为了研究该基因的功能，我们将ZmZLP 1与质膜定位蛋白AtIRT 1的信号肽融合，并将该融合蛋白转化到锌摄取缺陷型酵母（酿酒酵母）菌株ZHY 3和野生型菌株DEY 1457中。IRT 1-ZmZLP 1转化体在锌限制培养基上生长不良，并且这种生长缺陷通过锌补充来挽救，这表明ZmZLP 1负责将锌从ER转运到细胞质。进一步的研究表明，ZmZLP 1参与未折叠蛋白反应（UPR）途径，并增强酵母细胞的耐热性。
The ZmZLP1 (ZmZIP-like protein) gene was isolated from a cDNA library of Zea mays L. (maize) pollen. Bioinformatics analysis indicated that ZmZLP1 shares many characteristics of the ZIP (ZRT/IRT-like protein) family of metal ion transporters. Under general nutrient conditions, the expression of ZmZLP1 was detected in both mature pollen and, less strongly, in male inflorescences, whereas an induction of the ZmZLP1 transcript was observed in roots after 12 h of zinc deprivation. The visualisation of GFP showed that ZmZLP1 was targeted to the endoplasmic reticulum (ER). To investigate the gene’s functions, we fused ZmZLP1 with the signal peptide of the plasma membrane-localised protein AtIRT1 and transformed this fusion protein into the zinc uptake-deficient yeast (Saccharomyces cerevisiae) strain ZHY3 and the wild-type strain DEY1457. The IRT1-ZmZLP1 transformants grew poorly on zinc-limited medium, and this growth defect was rescued by zinc supplementation, suggesting that ZmZLP1 is responsible for transporting zinc from the ER to the cytoplasm. Further research indicated that ZmZLP1 is involved in the unfolded protein response (UPR) pathway and enhances the heat resistance of yeast cells.
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影响因子：
1
作者：
R. Chauhan
通讯作者：
R. Chauhan
DOI：
10.1007/978-94-011-4217-5
发表时间：
2000
期刊：
--
影响因子：
--
作者：
S. Gelvin;R. Schilperoort
通讯作者：
S. Gelvin;R. Schilperoort
影响因子：
7.4
作者：
Ramesh, SA;Shin, R;Schachtman, DP
通讯作者：
Schachtman, DP
影响因子：
11.6
作者：
Vert, G;Grotz, N;Curie, C
通讯作者：
Curie, C
影响因子：
7.8
作者：
Ellis, Charissa D;Wang, Fudi;MacDiarmid, Colin W;Clark, Suzanne;Lyons, Thomas;Eide, David J
通讯作者：
Eide, David J