Mechanisms contributing to fluid‐flow‐induced Ca2+ mobilization in articular chondrocytes
Mechanisms contributing to fluid‐flow‐induced Ca2+ mobilization in articular chondrocytes
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关节软骨细胞中流体流动诱导 Ca2+ 动员的机制
DOI：
--
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发表时间：
1999
影响因子：
5.6
通讯作者：
H. Donahue
中科院分区：
文献类型：
--
作者：
Clare E. Yellowley;C. Jacobs;H. Donahue
我们以前表明，流体流动，软骨细胞在体内经历，并在培养的关节软骨细胞的各种形态和代谢变化的结果，也可以刺激细胞内钙浓度（[Ca 2 +]i）的上升。然而，Ca 2+响应流动而动员的机制尚不清楚。在这项研究中，我们研究了细胞内Ca 2+储存，G蛋白和细胞外ATP在牛关节软骨细胞（BAC）流动诱导的Ca 2+反应中的作用。将负载有Ca 2+敏感染料Fura-2的细胞在平行板流动室中暴露于34 ml/min（37达因/cm 2）的稳定流动。而ryanodine和咖啡因没有影响，新霉素和毒胡萝卜素都显着降低了Ca 2 +i对流量的反应，表明Ca 2+库释放的作用，可能通过肌醇1，4，5-三磷酸（IP 3）依赖性机制。用百日咳毒素处理24小时也显著降低了反应，表明该机制可能是G蛋白调节的。此外，软骨细胞释放的ATP似乎不介导流动诱导的Ca 2+反应，因为苏拉明（一种P2嘌呤能阻断剂）没有影响。这些结果表明，BAC通过涉及IP 3刺激的Ca 2 +i释放和G蛋白活化的机制对机械环境的变化（例如增加的流体流量）迅速作出反应。J.细胞。180：402-408，1999。© 1999 Wiley利斯公司
We previously showed that fluid flow, which chondrocytes experience in vivo and which results in a variety of morphological and metabolic changes in cultured articular chondrocytes, can also stimulate a rise in intracellular calcium concentration ([Ca2+]i). However, the mechanism by which Ca2+ is mobilized in response to flow is unclear. In this study, we investigated the roles of intracellular Ca2+ stores, G‐proteins, and extracellular ATP in the flow‐induced Ca2+ response in bovine articular chondrocytes (BAC). Cells loaded with the Ca2+ sensitive dye Fura‐2 were exposed to steady flow at 34 ml/min (37 dynes/cm2) in a parallel plate flow chamber. Whereas ryanodine and caffeine had no effect, both neomycin and thapsigargin significantly decreased the Ca2+i response to flow, suggesting a role for Ca2+ store release, possibly through an inositol 1,4,5‐trisphosphate (IP3)‐dependent mechanism. Twenty‐four‐hour treatment with pertussis toxin also significantly decreased the response, suggesting that the mechanism may be G‐protein regulated. In addition, ATP release by chondrocytes does not appear to mediate the flow‐induced Ca2+ response because suramin, a P2 purinergic blocker, had no effect. These results suggest that BAC respond rapidly to changes in their mechanical environment, such as increased fluid flow, by a mechanism that involves IP3 stimulated Ca2+i release and G‐protein activation. J. Cell. Physiol. 180:402–408, 1999. © 1999 Wiley‐Liss, Inc.
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DOI：
10.1152/ajpcell.1997.273.1.c30
发表时间：
1997
期刊：
The American journal of physiology.
影响因子：
--
作者：
Yellowley,CE;Jacobs,CR;Li,Z;Zhou,Z;Donahue,HJ
通讯作者：
Donahue,HJ
影响因子：
2.4
作者：
Hung, CT;Allen, FD;Brighton, CT
通讯作者：
Brighton, CT
影响因子：
2.4
作者：
Jacobs, CR;Yellowley, CE;Donahue, HJ
通讯作者：
Donahue, HJ
影响因子：
4.8
作者：
K. M. Reich;Todd N. McAllister;S. Gudi;J. Frangos
通讯作者：
K. M. Reich;Todd N. McAllister;S. Gudi;J. Frangos
影响因子：
1.7
作者：
BHAGYALAKSHMI, A;BERTHIAUME, F;FRANGOS, JA
通讯作者：
FRANGOS, JA