High tyrosine kinase activity in normal nonproliferating cells
High tyrosine kinase activity in normal nonproliferating cells
复制标题
正常非增殖细胞中的高酪氨酸激酶活性
作者：
F. Tuy;J. Henry;C. Rosenfeld;A. Kahn
丝氨酸和苏氨酸残基处的蛋白质磷酸化已经涉及许多细胞过程的调节。最近，酪氨酸残基磷酸化已显示与细胞增殖的刺激有关，包括病毒转化1 - 7和表皮生长因子（EGF）7 - 10、血小板衍生生长因子（PDGF）10 - 12和与细胞生长相关的其它化合物如胰岛素13 - 15和二甲基亚砜16的刺激。为了比较正常和白血病人造血细胞在体内和体外的蛋白激酶和磷蛋白，我们首先研究了磷酸丝氨酸，磷酸苏氨酸和磷酸酪氨酸的百分比水解后获得的蛋白质从不同的血细胞组分在体外磷酸化。我们在此报道，磷酸酪氨酸形成来自多形核细胞、单核细胞（80%循环淋巴细胞，20%单核细胞）、血小板和红细胞（未显示）的可溶性级分的小于1%。令人惊讶的是，仅在来自非增殖无核细胞、血小板和红细胞的颗粒级分中发现高百分比的磷酸化酪氨酸。
Protein phosphorylation at serine and threonine residues has been implicated in the regulation of many cellular processes. More recently, tyrosine residue phosphorylation has been shown to be associated with stimulation of cell proliferation, including viral transformation1–7 and stimulation by epidermal growth factors (EGF)7–10, platelet-derived growth factor (PDGF)10–12 and other compounds related to cellular growth such as insulin13–15 and dimethyl sulphoxide16. To compare protein kinases and phosphoproteins of normal and leukaemic human haematopoietic cells in vivo and in vitro, we first have investigated the percentages of phosphoserine, phosphothreonine and phosphotyrosine obtained after hydrolysis of proteins from different blood cell fractions phosphorylated in vitro. We report here that phosphotyrosine formed less than 1% of the soluble fractions from polymorphonuclear cells, mononuclear cells (80% circulating lymphocytes, 20% monocytes), blood platelets and red blood cells (not shown). Surprisingly, high percentages of phosphorylated tyrosine were found only in the particulate fractions from non-proliferating anuclear cells, platelets and red blood cells.
二甲亚砜刺激大鼠肝表皮生长因子受体酪氨酸残基磷酸化。
DOI：
10.1126/science.6294827
发表时间：
1983
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rubin,RA;Earp,HS
通讯作者：
Earp,HS
人血小板衍生生长因子刺激瑞士小鼠 3T3 细胞中的蛋白质磷酸化。
DOI：
10.1016/0006-291x(81)91700-9
发表时间：
1981
影响因子：
3.1
作者：
Nishimura,J;Deuel,TF
通讯作者：
Deuel,TF
DOI：
10.1073/pnas.78.10.6013
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SMART, JE;OPPERMANN, H;BISHOP, JM
通讯作者：
BISHOP, JM