Mitochondrial Uncoupling Protein 1 Overexpression Increases Yield in Nicotiana tabacum under Drought Stress by Improving Source and Sink Metabolism.
Mitochondrial Uncoupling Protein 1 Overexpression Increases Yield in Nicotiana tabacum under Drought Stress by Improving Source and Sink Metabolism.
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DOI：
10.3389/fpls.2017.01836
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发表时间：
2017
影响因子：
5.6
通讯作者：
Arruda P
中科院分区：
文献类型：
--
作者：
Barreto P;Yassitepe JECT;Wilson ZA;Arruda P
线粒体解偶联蛋白 (UCP) 维持线粒体呼吸，与细胞内 ATP 浓度无关。解耦呼吸在应激条件下特别有益，在此期间光合作用和呼吸可能都会受到损害。在繁殖阶段维持碳固定对于植物发育有活力的花粉粒和结籽至关重要。在这里，我们研究了 UCP1 过度表达 (UCP1-oe) 是否有助于烟草植物应对生殖发育过程中的干旱胁迫。我们观察到，在中度干旱胁迫下，WT 和 UCP1-oe 植物失水的速度相同，但 UCP1-oe 品系在重新浇水时恢复水分的速度更快。 UCP1-oe植物在干旱胁迫期间保持较高水平的呼吸和光合作用，并降低叶片中的H2O2含量。我们通过监测花发育的进展（重点关注花粉形成的早期阶段）来检查 UCP1-oe 是否影响生殖组织和种子生产。 UCP1-oe品系诱导线粒体基因的表达并增加生殖组织中的线粒体DNA含量，从而增加碳水化合物的消耗并减少H2O2含量和花粉干扰。最后，UCP1-oe 对源和库器官的有益影响导致在控制条件和干旱胁迫下种子尺寸和数量增加。
Mitochondrial uncoupling proteins (UCPs) sustain mitochondrial respiration independent of intracellular ATP concentration. Uncoupled respiration is particularly beneficial under stress conditions, during which both photosynthesis and respiration may be impaired. Sustaining carbon fixation during the reproductive phase is essential for plants to develop viable pollen grains and for seed setting. Here, we examined whether UCP1 overexpression (UCP1-oe) would help tobacco plants cope with drought stress during reproductive development. We observed that WT and UCP1-oe plants lost water at the same rate under moderate drought stress, but that UCP1-oe lines regained water faster upon rewatering. UCP1-oe plants maintained higher levels of respiration and photosynthesis and decreased H2O2 content in the leaves during the drought stress period. We examined whether UCP1-oe impacts reproductive tissues and seed production by monitoring the progress of flower development, focusing on the early stages of pollen formation. UCP1-oe lines induced the expression of mitochondrial genes and increased mtDNA content in reproductive tissues, which increased the consumption of carbohydrates and reduced H2O2 content and pollen disturbances. Finally, the beneficial impact of UCP1-oe on the source and sink organs resulted in an increased seed size and number under both control conditions and drought stress.
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影响因子：
11.6
作者：
Araujo, Wagner L.;Nunes-Nesi, Adriano;Fernie, Alisdair R.
通讯作者：
Fernie, Alisdair R.
影响因子：
6.9
作者：
Dahal K;Martyn GD;Alber NA;Vanlerberghe GC
通讯作者：
Vanlerberghe GC
影响因子：
7.4
作者：
Andersen, MN;Asch, F;Koch, KE
通讯作者：
Koch, KE
影响因子：
9.4
作者：
Dahal, Keshav;Martyn, Greg D.;Vanlerberghe, Greg C.
通讯作者：
Vanlerberghe, Greg C.
影响因子：
7.4
作者：
Claeys, Hannes;Van Landeghem, Sofie;Inze, Dirk
通讯作者：
Inze, Dirk