High-speed 3D imaging of cellular activity in the brain using axially-extended beams and light sheets.
High-speed 3D imaging of cellular activity in the brain using axially-extended beams and light sheets.
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DOI：
10.1016/j.conb.2018.03.007
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发表时间：
2018-06
影响因子：
5.7
通讯作者：
Galwaduge PT
中科院分区：
文献类型：
--
作者：
Hillman EM;Voleti V;Patel K;Li W;Yu H;Perez-Campos C;Benezra SE;Bruno RM;Galwaduge PT
随着细胞活动的光学报告者和调节器变得越来越复杂，通过高速细胞成像可以了解大脑的数量急剧增加。然而，尽管有热情的创新，点扫描双光子显微镜在可实现的3D成像速度和视野方面面临着根本性的限制。一系列替代方法正在出现，其中一些正在远离点扫描，以使用轴向延伸的光束或光片，例如扫描共焦对准平面激发（SCAPE）显微镜。这些方法被证明是有效的高速体积成像的神经系统的小生物，如果蝇（果蝇）和D。Rerio（斑马鱼），并显示出使用单光子和双光子激发在活体哺乳动物大脑中成像活动的前景。本文介绍了这些方法，并提出了一个简单的模型，展示了轴向扩展照明的点扫描策略的高速体积成像的关键优势，包括更长的积分时间每体素，提高光子效率和减少光损伤。
As optical reporters and modulators of cellular activity have become increasingly sophisticated, the amount that can be learned about the brain via high-speed cellular imaging has increased dramatically. However, despite fervent innovation, point-scanning two-photon microscopy is facing a fundamental limit in achievable 3D imaging speeds and fields of view. A range of alternative approaches are emerging, some of which are moving away from point-scanning to use axially-extended beams or sheets of light, for example swept confocally aligned planar excitation (SCAPE) microscopy. These methods are proving effective for high-speed volumetric imaging of the nervous system of small organisms such as Drosophila (fruit fly) and D. Rerio (Zebrafish), and are showing promise for imaging activity in the living mammalian brain using both single and two-photon excitation. This article describes these approaches and presents a simple model that demonstrates key advantages of axially-extended illumination over point-scanning strategies for high-speed volumetric imaging, including longer integration times per voxel, improved photon efficiency and reduced photodamage.
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