Imaging neural spiking in brain tissue using FRET-opsin protein voltage sensors.
Imaging neural spiking in brain tissue using FRET-opsin protein voltage sensors.
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DOI：
10.1038/ncomms4674
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发表时间：
2014-04-22
影响因子：
16.6
通讯作者：
Schnitzer, Mark J.
中科院分区：
文献类型：
--
作者：
Gong, Yiyang;Wagner, Mark J.;Li, Jin Zhong;Schnitzer, Mark J.
遗传编码的荧光电压传感器提供了直接可视化细胞中神经尖峰动态的可能性，这些细胞由其遗传类别或连接性作为目标。这类传感器通常遭受适度亮度、缓慢动力学和响应于动作电位的有限信号动态范围之间的性能限制权衡。在这里，我们描述的传感器，使用荧光共振能量转移（FRET）的快速动力学和大量的视紫红质家族的电压敏感域的电压依赖性与遗传工程蛋白质荧光团的亮度结合联合收割机。这些FRET视蛋白传感器显著提高了由遗传编码电压传感器Arclight提供的尖峰检测保真度，同时提供更快的动力学和更高的亮度。使用FRET-视蛋白传感器，我们在培养的神经元和新皮层组织切片内的锥体细胞中成像神经尖峰和阈下膜电压动态。在活体小鼠中，小脑浦肯野神经元树突电压瞬变的速率和光学波形与这些细胞树突棘波的预期相匹配。
Genetically encoded fluorescence voltage sensors offer the possibility of directly visualizing neural spiking dynamics in cells targeted by their genetic class or connectivity. Sensors of this class have generally suffered performance-limiting tradeoffs between modest brightness, sluggish kinetics, and limited signaling dynamic range in response to action potentials. Here we describe sensors that use fluorescence resonance energy transfer (FRET) to combine the rapid kinetics and substantial voltage-dependence of rhodopsin family voltage-sensing domains with the brightness of genetically engineered protein fluorophores. These FRET-opsin sensors significantly improve upon the spike detection fidelity offered by the genetically encoded voltage sensor, Arclight, while offering faster kinetics and higher brightness. Using FRET-opsin sensors we imaged neural spiking and sub-threshold membrane voltage dynamics in cultured neurons and in pyramidal cells within neocortical tissue slices. In live mice, rates and optical waveforms of cerebellar Purkinje neurons’ dendritic voltage transients matched expectations for these cells’ dendritic spikes.
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影响因子：
3.7
作者：
Gong Y;Li JZ;Schnitzer MJ
通讯作者：
Schnitzer MJ
影响因子：
16.2
作者：
Jin L;Han Z;Platisa J;Wooltorton JR;Cohen LB;Pieribone VA
通讯作者：
Pieribone VA
影响因子：
16.2
作者：
Grienberger, Christine;Konnerth, Arthur
通讯作者：
Konnerth, Arthur
影响因子：
48
作者：
Kralj, Joel M.;Douglass, Adam D.;Hochbaum, Daniel R.;Maclaurin, Dougal;Cohen, Adam E.
通讯作者：
Cohen, Adam E.
DOI：
10.1073/pnas.93.21.11618
发表时间：
1996-10-15
影响因子：
11.1
作者：
Kolodner, P;Lukashev, EP;Rousseau, DL
通讯作者：
Rousseau, DL