Novel DNA vaccine based on hepatitis B virus core gene induces specific immune responses in Balb/c mice.
Novel DNA vaccine based on hepatitis B virus core gene induces specific immune responses in Balb/c mice.
复制标题
基于乙型肝炎病毒核心基因的新型DNA疫苗在Balb/c小鼠中诱导特异性免疫反应。
DOI：
10.3748/wjg.v11.i29.4583
复制
发表时间：
2005
影响因子：
4.3
通讯作者：
Shan Lu
中科院分区：
文献类型：
--
作者：
Yi;Zu;Shixia Wang;Jie Cai;Jun Li;Te;Shan Lu
目的
AIM
To investigate the immunogenicity of a novel DNA vaccine, pSW3891/HBc, based on HBV core gene in Balb/c mice.
METHODS
A novel DNA vaccine, pSW3891/HBc, encoding HBV core gene was constructed using a vector plasmid pSW3891. Balb/c mice were immunized with either pSW3891/HBc or empty vector DNA via gene gun. IgG anti-HBc responses in mouse sera were demonstrated by ELISA. Specific cytotoxicity of cytotoxic T lymphocytes (CTLs) of mice was quantitatively measured by lactate dehydrogenase release assay.
RESULTS
HBcAg was expressed effectively in 293T cell line transiently transfected with pSW3891/HBc. Strong IgG anti-HBc responses were elicited in mice immunized with pSW3891/HBc. The end-point titers of anti-HBc reached the highest 1:97,200, 4 wk after the third immunization. The specific CTL killing with the highest specific lysis reached 73.25% at effector:target ratio of 20:1 in mice that received pSW3891/HBc DNA vaccine.
CONCLUSION
pSW3891/HBc vaccination elicits specific anti-HBc response and induces HBc-specific CTL response in immunized Balb/c mice.
影响因子：
4.4
作者：
C. Ferrari;A. Penna;A. Bertoletti;A. Valli;A. Antoni;T. Giuberti;A. Cavalli;M. Petit;F. Fiaccadori
通讯作者：
C. Ferrari;A. Penna;A. Bertoletti;A. Valli;A. Antoni;T. Giuberti;A. Cavalli;M. Petit;F. Fiaccadori
DOI：
10.1073/pnas.93.14.7213
发表时间：
1996-07-09
影响因子：
11.1
作者：
Davis, HL;McCluskie, MJ;Purcell, RH
通讯作者：
Purcell, RH