Enhanced nanochannel translocation and localization of genomic DNA molecules using three-dimensional nanofunnels.
Enhanced nanochannel translocation and localization of genomic DNA molecules using three-dimensional nanofunnels.
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DOI：
10.1038/s41467-017-00951-4
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发表时间：
2017-10-09
影响因子：
16.6
通讯作者：
Ramsey JM
中科院分区：
文献类型：
--
作者：
Zhou J;Wang Y;Menard LD;Panyukov S;Rubinstein M;Ramsey JM
精确控制单个DNA分子通过纳米级通道的运输的能力对于目前正在开发的DNA测序和制图技术至关重要。在这里，我们展示了如何通过在纳米通道入口加入三维纳米通道来促进电动驱动的DNA分子导入纳米通道。当单个DNA分子试图通过不同形状的纳米通道克服纳米通道进入的熵障时，就会被成像。这种行为的理论模型揭示了推动和拉动的力量，导致启动纳米通道进入所需的阈值电场减少了30倍。在某些情况下，DNA分子在亚阈值电场强度下被稳定地捕获并轴向定位在纳米通道内，这表明纳米通道作为力谱工具的效用。这些应用说明了精细调整纳米尺度导管几何形状的好处，可以使用这里开发的理论模型来设计。迫使DNA分子进入纳米级通道需要克服与限制有关的自由能垒。在这里，作者展示了通过漏斗形入口注入的DNA更有效地进入纳米通道，这要归功于纳米通道几何形状产生的促进力。
The ability to precisely control the transport of single DNA molecules through a nanoscale channel is critical to DNA sequencing and mapping technologies that are currently under development. Here we show how the electrokinetically driven introduction of DNA molecules into a nanochannel is facilitated by incorporating a three-dimensional nanofunnel at the nanochannel entrance. Individual DNA molecules are imaged as they attempt to overcome the entropic barrier to nanochannel entry through nanofunnels with various shapes. Theoretical modeling of this behavior reveals the pushing and pulling forces that result in up to a 30-fold reduction in the threshold electric field needed to initiate nanochannel entry. In some cases, DNA molecules are stably trapped and axially positioned within a nanofunnel at sub-threshold electric field strengths, suggesting the utility of nanofunnels as force spectroscopy tools. These applications illustrate the benefit of finely tuning nanoscale conduit geometries, which can be designed using the theoretical model developed here. Forcing a DNA molecule into a nanoscale channel requires overcoming the free energy barrier associated with confinement. Here, the authors show that DNA injected through a funnel-shaped entrance more efficiently enters the nanochannel, thanks to facilitating forces generated by the nanofunnel geometry.
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影响因子：
4.6
作者：
Ohshiro, Takahito;Matsubara, Kazuki;Tsutsui, Makusu;Furuhashi, Masayuki;Taniguchi, Masateru;Kawai, Tomoji
通讯作者：
Kawai, Tomoji
影响因子：
10.8
作者：
McNally B;Singer A;Yu Z;Sun Y;Weng Z;Meller A
通讯作者：
Meller A
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
4
作者：
Cao, H;Tegenfeldt, JO;Chou, SY
通讯作者：
Chou, SY
影响因子：
3.3
作者：
de Zoysa, Ranulu Samanthi S.;Jayawardhana, Dilani A.;Guan, Xiyun
通讯作者：
Guan, Xiyun