High-Field Dynamic Nuclear Polarization for Solid and Solution Biological NMR.
High-Field Dynamic Nuclear Polarization for Solid and Solution Biological NMR.
复制标题
固体和溶液生物NMR的高场动态核极化。
DOI：
10.1007/s00723-008-0129-1
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发表时间：
2008-08
影响因子：
1
通讯作者：
Griffin, R. G.
中科院分区：
文献类型：
--
作者：
Barnes, A. B.;De Paepe, G.;van der Wel, P. C. A.;Hu, K. -N.;Joo, C. -G.;Bajaj, V. S.;Mak-Jurkauskas, M. L.;Sirigiri, J. R.;Herzfeld, J.;Temkin, R. J.;Griffin, R. G.
动态核极化（DNP）通过将磁化从电子转移到原子核而导致核极化增强。近年来，DNP实验的发展取得了相当大的进展，旨在提高生物核磁共振（NMR）的灵敏度。本文综述了麻省理工学院Francis Bitter磁体实验室为高场DNP实验开发的应用、硬件、极化剂和理论描述。在冷冻介质中，极化剂附近的核极化增强可以通过1H自旋扩散有效地分散到样品中。该策略已被证明在极化生物感兴趣的系统中是有效的，如纳米晶肽和膜蛋白，而不会导致核磁共振信号的顺磁展宽。在DNP实验中，回旋管被用作高达460 GHz的高功率（5-10 W）微波源。其他硬件也被开发出来，使原位微波辐射与低温魔角旋转固体核磁共振相结合。量子力学处理的进展成功地描述了新的双基极化剂在更高磁场下产生更大增强的机制。最后，脉冲方法和溶液实验将在未来的DNP研究中发挥重要作用。
Dynamic nuclear polarization (DNP) results in a substantial nuclear polarization enhancement through a transfer of the magnetization from electrons to nuclei. Recent years have seen considerable progress in the development of DNP experiments directed towards enhancing sensitivity in biological nuclear magnetic resonance (NMR). This review covers the applications, hardware, polarizing agents, and theoretical descriptions that were developed at the Francis Bitter Magnet Laboratory at Massachusetts Institute of Technology for high-field DNP experiments. In frozen dielectrics, the enhanced nuclear polarization developed in the vicinity of the polarizing agent can be efficiently dispersed to the bulk of the sample via 1H spin diffusion. This strategy has been proven effective in polarizing biologically interesting systems, such as nanocrystalline peptides and membrane proteins, without leading to paramagnetic broadening of the NMR signals. Gyrotrons have been used as a source of high-power (5–10 W) microwaves up to 460 GHz as required for the DNP experiments. Other hardware has also been developed allowing in situ microwave irradiation integrated with cryogenic magic-angle-spinning solid-state NMR. Advances in the quantum mechanical treatment are successful in describing the mechanism by which new biradical polarizing agents yield larger enhancements at higher magnetic fields. Finally, pulsed methods and solution experiments should play a prominent role in the future of DNP.
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影响因子：
4.4
作者：
BENNETT, AE;RIENSTRA, CM;GRIFFIN, RG
通讯作者：
GRIFFIN, RG
影响因子：
4.4
作者：
Hu, Kan-Nian;Bajaj, Vikram S.;Griffin, Robert G.
通讯作者：
Griffin, Robert G.
影响因子：
2.8
作者：
DORN, HC;GITTI, R;GLASS, TE
通讯作者：
GLASS, TE
影响因子：
56.9
作者：
Hall, DA;Maus, DC;Griffin, RG
通讯作者：
Griffin, RG
DOI：
10.1073/pnas.252644399
发表时间：
2002-12-10
影响因子：
11.1
作者：
Frydman, L;Scherf, T;Lupulescu, A
通讯作者：
Lupulescu, A