Effect of Lactobacillus rhamnosus GG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome.
Effect of Lactobacillus rhamnosus GG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome.
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鼠李糖乳杆菌GG上清液对感染后肠易激综合征大鼠血清素转运蛋白表达的影响
DOI：
10.3748/wjg.v24.i3.338
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发表时间：
2018-01-21
影响因子：
4.3
通讯作者：
Wang YM
中科院分区：
文献类型：
--
作者：
Cao YN;Feng LJ;Liu YY;Jiang K;Zhang MJ;Gu YX;Wang BM;Gao J;Wang ZL;Wang YM
目的探讨鼠李糖乳杆菌GG培养上清液（LGG-s）对感染后肠易激综合征（PI-IBS）大鼠血清素转运体（SERT）表达的影响。方法用空肠弯曲菌81-176（10 ~（10）CFU/mL）诱导大鼠肠道感染，建立PI-IBS模型。在通过生化试验、DNA琼脂糖凝胶电泳、腹部退缩反射（AWR）试验和肠动力试验评估感染后阶段后，四个PI-IBS组接受不同浓度的LGG-s，持续4周。在实验期间，处理维持1.0、2.0、3.0或4.0 wk，并且每周取出结肠和脑以供以后使用。实时荧光定量PCR和Western blot检测SERT mRNA和蛋白水平。结果LGG-s组大鼠肠组织SERT mRNA和蛋白水平均高于对照组和PBS组。与对照组相比，未稀释的LGG-s在第2周时使SERT mRNA的表达量增加了2.67倍，此后SERT mRNA的表达量继续增加。双倍稀释的LGG-s与未稀释的LGG-s相似，导致高水平的SERT mRNA。与对照组相比，三倍稀释的LGG-s使SERT mRNA表达水平上调6.9倍，但SERT mRNA表达在第二周末迅速下降。在第一周，未稀释的LGG-s、二倍稀释的LGG-s和三倍稀释的LGG-s处理的大鼠的SERT蛋白水平基本相当，高于对照组和PBS处理的PI-IBS组。在第二周和第三周，用未稀释的LGG-s、两倍稀释的LGG-s和三倍稀释的LGG-s处理的大鼠中，肠中的SERT蛋白水平也相当。实验期间各组脑组织SERT mRNA和蛋白水平无统计学差异。结论LGG-s可上调PI-IBS大鼠肠组织SERT mRNA和蛋白水平，但对脑组织无影响。
AIM To evaluate the effect of Lactobacillus rhamnosus GG supernatant (LGG-s) on the expression of serotonin transporter (SERT) in rats with post-infectious irritable bowel syndrome (PI-IBS). METHODS Campylobacter jejuni 81-176 (1010 CFU/mL) was used to induce intestinal infection to develop a PI-IBS model. After evaluation of the post-infectious phase by biochemical tests, DNA agarose gel electrophoresis, abdominal withdrawal reflex (AWR) test, and the intestinal motility test, four PI-IBS groups received different concentrations of LGG-s for 4 wk. The treatments were maintained for 1.0, 2.0, 3.0 or 4.0 wk during the experiment, and the colons and brains were removed for later use each week. SERT mRNA and protein levels were detected by real-time PCR and Western blot, respectively. RESULTS The levels of SERT mRNA and protein in intestinal tissue were higher in rats treated with LGG-s than in control rats and PI-IBS rats gavaged with PBS during the whole study. Undiluted LGG-s up-regulated SERT mRNA level by 2.67 times compared with the control group by week 2, and SERT mRNA expression kept increasing later. Double-diluted LGG-s was similar to undiluted-LGG-s, resulting in high levels of SERT mRNA. Triple-diluted LGG-s up-regulated SERT mRNA expression level by 6.9-times compared with the control group, but SERT mRNA expression decreased rapidly at the end of the second week. At the first week, SERT protein levels were basically comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triple-diluted LGG-s, which were higher than those in the control group and PBS-treated PI-IBS group. SERT protein levels in the intestine were also comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triple-diluted LGG-s by the second and third weeks. SERT mRNA and protein levels in the brain had no statistical difference in the groups during the experiment. CONCLUSION LGG-s can up-regulate SERT mRNA and protein levels in intestinal tissue but has no influence in brain tissue in rats with PI-IBS.
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影响因子：
8
作者：
Francavilla, Ruggiero;Miniello, Vito;Cavallo, Luciano
通讯作者：
Cavallo, Luciano
DOI：
10.3390/ph7100999
发表时间：
2014-10-10
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
Floch MH
通讯作者：
Floch MH
影响因子：
29.4
作者：
Faure C;Patey N;Gauthier C;Brooks EM;Mawe GM
通讯作者：
Mawe GM
影响因子：
9.8
作者：
Cremon, Cesare;Carini, Giovanni;Barbara, Giovanni
通讯作者：
Barbara, Giovanni
影响因子：
5.3
作者：
Chen, JJ;Li, ZS;Gershon, MD
通讯作者：
Gershon, MD