Serotonin signaling is altered in irritable bowel syndrome with diarrhea but not in functional dyspepsia in pediatric age patients.
Serotonin signaling is altered in irritable bowel syndrome with diarrhea but not in functional dyspepsia in pediatric age patients.
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DOI：
10.1053/j.gastro.2010.03.032
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发表时间：
2010-07
期刊：
影响因子：
29.4
通讯作者：
Mawe GM
中科院分区：
文献类型：
--
作者：
Faure C;Patey N;Gauthier C;Brooks EM;Mawe GM
在成年人中，肠易激综合征(IBS)和功能性消化不良(FD)是一种慢性疾病，通常始于儿童时期。我们研究了儿童的粘膜5-羟色胺(5-HT)信号，认为来自病史较短的受试者的数据可能会提高我们对潜在病理生理机制的理解。对98名接受胃镜或结肠镜检查的儿童进行前瞻性研究。活检标本的炎症、肠嗜铬细胞数、5-羟色胺含量以及合成酶色氨酸羟基酶1(TPH1)和5-羟色胺转运体(SERT)的mRNA水平通过实时定量RT-PCR进行评估。将12名IBS儿童和17名FD儿童的数据与年龄匹配的对照组(12名直肠活检和12名胃活检)和器质性疾病的受试者进行比较。在FD患者中，半数患者的胃粘膜可见少量免疫细胞，但未发现5-羟色胺途径的异常。在IBS患者中，尽管所有患者都表现出至少轻微的炎症浸润性，但患者和对照组之间在固有层上皮内淋巴细胞和CD3+细胞方面没有发现差异。在IBS患者中，5-羟色胺含量高于对照组(P<；0.01)，SERT基因表达水平低于对照组(P<；0.05)。结肠粘膜的严重炎症对5-羟色胺信号转导有很大影响，EC细胞显著减少(P<；0.01)，5-羟色胺含量显著降低(P<；0.01)，SERT mRNA表达增加(P<；0.01)。这些结果证实了5-羟色胺信号在儿童IBS中的作用，并反对这种作用在FD中的作用。
In adults, irritable bowel syndrome (IBS) and functional dyspepsia (FD) are chronic conditions that often start during childhood. We investigated mucosal serotonin (5-HT) signalling in children with the idea that data from subjects with a shorter history may improve our understanding of underlying pathophysiological mechanisms. 98 children undergoing gastroscopy or colonoscopy were prospectively studied. Biopsy specimens were evaluated for inflammation, enterochromaffin cell numbers, 5-HT content, and mRNA levels for the synthetic enzyme, tryptophan hydroxylase 1 (TpH1) and the serotonin transporter (SERT) were assessed by quantitative real-time RT-PCR. Data from 12 children with IBS and 17 with FD were compared to age-matched controls (12 with rectal biopsies and 12 with gastric biopsies) and to subjects with organic disorders. In patients with FD, a small number of immune cells were observed in the gastric mucosa in half of the patients, but no abnormalities with respect of the 5-HT pathway were identified. In patients with IBS, no differences were detected between patients and controls regarding intraepithelial lymphocytes and CD3+ cells in the lamina propria although all patients exhibited at least a slight inflammatory infiltrate. In the IBS samples, higher 5-HT content (P<0.01) and lower SERT mRNA (P<0.05) were detected as compared to controls. Severe inflammation in the colonic mucosa had a high impact on 5-HT signalling with a significant decrease in EC cells (P<0.01) and 5-HT content (P<0.01) and a high SERT mRNA expression (P<0.01). These results confirm in children the role of 5-HT signalling in IBS and argue against such a role in FD.
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DOI：
10.1007/bf00712758
发表时间：
1987-01-01
期刊：
VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY
影响因子：
--
作者：
BISHOP, AE;PIETROLETTI, R;POLAK, JM
通讯作者：
POLAK, JM
影响因子：
8
作者：
Di Lorenzo, C;Colletti, RB;Walker, LS
通讯作者：
Walker, LS
影响因子：
5.1
作者：
Faure, Christophe;Wieckowska, Anna
通讯作者：
Wieckowska, Anna
影响因子：
11.1
作者：
El-Salhy, M;Danielsson, Å;Grimelius, L
通讯作者：
Grimelius, L
影响因子：
5.2
作者：
CHRISTENSEN, MF;MORTENSEN, O
通讯作者：
MORTENSEN, O