Identifying and Differentiating Topological G-Quadruplex Structures with DNA-Encoded Plasmonic Gold Nanoparticles.
Identifying and Differentiating Topological G-Quadruplex Structures with DNA-Encoded Plasmonic Gold Nanoparticles.
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DOI：
10.1002/anie.202204201
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发表时间：
2022-09-26
影响因子：
16.6
通讯作者：
Lu, Yi
中科院分区：
文献类型：
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作者：
Song, Tingjie;Wang, Xiaojing;Yao, Dongbao;Liang, Haojun;Lu, Yi
DNA g -四重体（G4s）已被确定为调节基因组功能和许多其他生物过程的关键元件。它们的功能高度依赖于一级核苷酸和二级折叠结构。因此，为了了解它们的功能，需要快速准确地识别和区分G4结构的方法，但这是有限的。在本报告中，我们应用合成的G4 DNA编码纳米颗粒方法来鉴定和区分具有不同拓扑结构和核苷酸残基的G4 DNA分子。我们发现，通过紫外可见光谱监测所得的金纳米粒子的等离子体性质，对不同的G4结构（包括堆积层、环序列、盖基和拓扑结构）非常敏感。通过这些系统的研究，我们证明了这种编码G4的金纳米粒子方法可以用来分析G4的结构，并将G4s与人类端粒区分开来。该方法在G4研究中具有广泛的应用前景。用dna编码的纳米颗粒生长策略来解决g -四重体（G4）结构。在该方法中，通过监测在G4存在下生长的等离子体金纳米粒子的紫外-可见消光，可以识别和区分G4 DNA中的拓扑结构和核苷酸残基。
DNA G-quadruplexes (G4s) have been identified as critical elements in modulating genomic functions and many other biological processes. Their functions are highly dependent on the primary nucleotides and secondary folding structures. Therefore, to understand their functions, methods to identify and differentiate structures of G4 with speed and accuracy are required but limited. In this report, we have applied a synthetic G4 DNA-encoded nanoparticle approach to identify and differentiate G4 DNA molecules with different topologies and nucleotide residues. We found that the resulting plasmonic properties of the gold nanoparticles, monitored by UV-vis spectroscopy, are quite sensitive to different G4 structures, including stacking layers, loop sequences, capping bases on G4s, and topological structures. Through these systematic investigations, we demonstrate that this G4-encoded gold nanoparticle approach can be used to profile the G4 structures and distinguish G4s from human telomeres. Such a method may have wide applications in G4 research. The G-quadruplex (G4) structures are resolved with DNA-encoded nanoparticle growth strategy. In this method, through monitoring the UV-vis extinction of plasmonic gold nanoparticle grown in the presence of G4s, both topological structures and nucleotide residues within G4 DNA, are identified and differentiated.
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