Hamster and ferret experimental infection with intranasal low dose of a single strain of SARS-CoV-2.
Hamster and ferret experimental infection with intranasal low dose of a single strain of SARS-CoV-2.
复制标题
低剂量单株SARS-CoV-2鼻内感染仓鼠和雪貂实验。
DOI：
10.1099/jgv.0.001567
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Marianneau P
中科院分区：
文献类型：
--
作者：
Monchatre-Leroy E;Lesellier S;Wasniewski M;Picard-Meyer E;Richomme C;Boué F;Lacôte S;Murri S;Pulido C;Vulin J;Salguero FJ;Gouilh MA;Servat A;Marianneau P
了解SARS-CoV-2感染的发病机制是制定针对COVID-19的预防和治疗策略的关键，无论是在重症病例中，还是在病情较轻的情况下。适当的实验动物模型的使用仍然是感染的病理生理学和抗病毒策略的体内探索的核心。本研究描述了SARS-CoV-2鼻内感染雪貂和仓鼠低剂量的低传代SARS-CoV-2临床法国分离株UCN 19，描述了两种动物物种的感染水平，排泄，免疫反应和病理模式。个体感染103 p.f.u. SARS-CoV-2在仓鼠中诱导的疾病比在雪貂中更严重。在仓鼠的肺中检测到病毒RNA，但在雪貂和两个物种的大脑（嗅球和/或延髓）中未检测到病毒RNA。总体而言，临床疾病保持轻度，仓鼠和雪貂分别在接种后7天和10天检测到血清学应答。病毒变得不可检测，病理学在14天内消退。感染的动力学和水平可以在雪貂和仓鼠中用作理解SARS-CoV-2的致病性和测试药物保护作用的实验模型。
Understanding the pathogenesis of the SARS-CoV-2 infection is key to developing preventive and therapeutic strategies against COVID-19, in the case of severe illness but also when the disease is mild. The use of appropriate experimental animal models remains central in the in vivo exploration of the physiopathology of infection and antiviral strategies. This study describes SARS-CoV-2 intranasal infection in ferrets and hamsters with low doses of low-passage SARS-CoV-2 clinical French isolate UCN19, describing infection levels, excretion, immune responses and pathological patterns in both animal species. Individual infection with 103 p.f.u. SARS-CoV-2 induced a more severe disease in hamsters than in ferrets. Viral RNA was detected in the lungs of hamsters but not of ferrets and in the brain (olfactory bulb and/or medulla oblongata) of both species. Overall, the clinical disease remained mild, with serological responses detected from 7 days and 10 days post-inoculation in hamsters and ferrets respectively. The virus became undetectable and pathology resolved within 14 days. The kinetics and levels of infection can be used in ferrets and hamsters as experimental models for understanding the pathogenicity of SARS-CoV-2, and testing the protective effect of drugs.
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影响因子：
28.3
作者：
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15.1
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