Determining the mechanical properties of human corneal basement membranes with atomic force microscopy.
Determining the mechanical properties of human corneal basement membranes with atomic force microscopy.
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DOI：
10.1016/j.jsb.2009.03.012
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发表时间：
2009-07
影响因子：
3
通讯作者：
Murphy, Christopher J.
中科院分区：
文献类型：
--
作者：
Last, Julie A.;Liliensiek, Sara J.;Nealey, Paul F.;Murphy, Christopher J.
生物物理线索，如底物模数，已被证明影响细胞的各种行为。用原子力显微镜(AFM)测定了人角膜前基底膜和后弹力膜的弹性模量。使用球形探头，其半径接近于典型细胞焦点粘连的半径。前基底膜的弹性模量值范围为2~15kPa，平均值为7.5±4.2kPa。Descemet膜的弹性模数略高于前基底膜，平均为50±17.8kPa，范围为20kPa80kPa.Descemet膜的形态与前基底膜相似，但孔径较小，结构更紧密。这种结构差异可能是观测到的模数差异的原因。这些值的确定将允许设计更好的细胞环境模型，并有助于人工角膜的设计和制造。
Biophysical cues such as substrate modulus have been shown to influence a variety of cell behaviors. We have determined the elastic modulus of the anterior basement membrane and Descemet’s membrane of the human cornea with atomic force microscopy (AFM). A spherical probe was used with a radius approximating that of a typical cell focal adhesion. Values obtained for the elastic modulus of the anterior basement membrane range from 2 kPa to 15 kPa, with a mean of 7.5 ± 4.2 kPa. The elastic modulus of Descemet’s membrane was found to be slightly higher than those observed for the anterior basement membrane, with a mean of 50 ± 17.8 kPa and a range of 20 kPa — 80 kPa. The topography of Descemet’s membrane has been shown to be similar to that of the anterior basement, but with smaller pore sizes resulting in a more tightly packed structure. This structural difference may account for the observed modulus differences. The determination of these values will allow for the design of a better model of the cellular environment as well as aid in the design and fabrication of artificial corneas.
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DOI：
10.1038/eye.1987.35
发表时间：
1987-01-01
期刊：
EYE-TRANSACTIONS OF THE OPHTHALMOLOGICAL SOCIETIES OF THE UNITED KINGDOM
影响因子：
--
作者：
FISHER, RF
通讯作者：
FISHER, RF
影响因子：
12.6
作者：
Jayasuriya, AC;Ghosh, S;Kramer, P
通讯作者：
Kramer, P
影响因子：
3.4
作者：
Engler, A;Bacakova, L;Discher, D
通讯作者：
Discher, D
影响因子：
4.9
作者：
Gunn, JW;Turner, SD;Mann, BK
通讯作者：
Mann, BK
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE