Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae.
Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae.
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DOI：
10.1073/pnas.2301358120
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发表时间：
2023-03-21
影响因子：
11.1
通讯作者：
Talbot, Nicholas J.
中科院分区：
文献类型：
--
作者：
Tang, Bozeng;Yan, Xia;Ryder, Lauren S.;Bautista, Mark Jave A.;Cruz-Mireles, Neftaly;Soanes, Darren M.;Molinari, Camilla;Foster, Andrew J.;Talbot, Nicholas J.
稻瘟病是全球粮食安全的主要威胁。稻瘟菌成功感染植物需要一系列效应蛋白来抑制植物免疫反应，从而实现植物组织的快速入侵。稻瘟菌在植物感染过程中如何调节效应子编码基因的表达尚不清楚。我们选择了错误调节效应子表达的突变体，并鉴定了一种新的调节因子 Rgs1，该调节因子先前与附着胞发育的控制有关。 Rgs1 在真菌感染植物细胞之前抑制效应基因转录，并在侵入性生长开始后抑制其表达。因此，稻瘟菌以复杂和动态的方式协调基因表达，以确保效应器在发病过程中的特定发育阶段部署。为了引起稻瘟病，丝状真菌稻瘟病菌将一系列效应蛋白分泌到宿主植物组织中以促进感染。效应子编码基因仅在植物感染期间表达，并且在其他发育阶段表现出非常低的表达。在米霉的侵入生长过程中，如何以如此精确的方式调节效应基因表达尚不清楚。在这里，我们报告了一种前向遗传筛选，以根据显示组成型效应基因表达的突变体的选择来识别效应基因表达的调节因子。通过这个简单的筛选，我们鉴定出 Rgs1（一种附着胞发育所必需的 G 蛋白信号传导 (RGS) 蛋白的调节因子）作为效应基因表达的新型转录调节因子，在植物感染之前发挥作用。我们发现，Rgs1 的 N 末端结构域具有反式激活活性，是效应基因调节所必需的，并且以不依赖于 RGS 的方式发挥作用。 Rgs1 控制至少 60 个时间共调节效应基因的表达，在植物感染之前的发育前渗透阶段阻止它们的转录。因此，在植物感染期间，米霉侵入性生长所需的病原体基因表达的协调也需要附着胞形态发生的调节剂。
Rice blast disease is a major threat to global food security. Successful plant infection by the blast fungus requires a battery of effector proteins to suppress plant immune responses, enabling the rapid invasion of plant tissue. How the blast fungus regulates expression of effector-encoding genes during plant infection is not known. We selected mutants that misregulated effector expression and identified a new regulator, Rgs1, previously implicated in control of appressorium development. Rgs1 represses effector gene transcription prior to the fungus infecting plant cells and derepresses their expression once invasive growth begins. The blast fungus therefore coordinates gene expression in a sophisticated and dynamic way to ensure that effectors are deployed at specific developmental stages during pathogenesis. To cause rice blast disease, the filamentous fungus Magnaporthe oryzae secretes a battery of effector proteins into host plant tissue to facilitate infection. Effector-encoding genes are expressed only during plant infection and show very low expression during other developmental stages. How effector gene expression is regulated in such a precise manner during invasive growth by M. oryzae is not known. Here, we report a forward-genetic screen to identify regulators of effector gene expression, based on the selection of mutants that show constitutive effector gene expression. Using this simple screen, we identify Rgs1, a regulator of G-protein signaling (RGS) protein that is necessary for appressorium development, as a novel transcriptional regulator of effector gene expression, which acts prior to plant infection. We show that an N-terminal domain of Rgs1, possessing transactivation activity, is required for effector gene regulation and acts in an RGS-independent manner. Rgs1 controls the expression of at least 60 temporally coregulated effector genes, preventing their transcription during the prepenetration stage of development prior to plant infection. A regulator of appressorium morphogenesis is therefore also required for the orchestration of pathogen gene expression required for invasive growth by M. oryzae during plant infection.
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DOI：
10.1007/s10482-017-0952-1
发表时间：
2018-03
期刊：
Antonie van Leeuwenhoek
影响因子：
--
作者：
Aerts D;Hauer EE;Ohm RA;Arentshorst M;Teertstra WR;Phippen C;Ram AFJ;Frisvad JC;Wösten HAB
通讯作者：
Wösten HAB
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
3.5
作者：
Hasegawa, Morifumi;Mitsuhara, Ichiro;Ohashi, Yuko
通讯作者：
Ohashi, Yuko
影响因子：
6.7
作者：
de Guillen K;Ortiz-Vallejo D;Gracy J;Fournier E;Kroj T;Padilla A
通讯作者：
Padilla A
影响因子：
4.8
作者：
Chatterjee, TK;Liu, ZY;Fisher, RA
通讯作者：
Fisher, RA