The Marek's disease virus (MDV) protein encoded by the UL17 ortholog is essential for virus growth.
The Marek's disease virus (MDV) protein encoded by the UL17 ortholog is essential for virus growth.
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DOI：
10.1051/vetres/2009012
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发表时间：
2009-07
影响因子：
4.4
通讯作者：
Vautherot JF
中科院分区：
文献类型：
--
作者：
Chbab N;Chabanne-Vautherot D;Francineau A;Osterrieder N;Denesvre C;Vautherot JF
马立克氏病病毒1型（MDV-1）在细胞培养中的繁殖严格依赖于直接的细胞间传播。由于MDV-1在细胞培养物中显示出受损的核出口，我们希望解决可能干预核内衣壳成熟的衣壳/被膜基因的表征。UL 17的直系同源物存在于所有疱疹病毒中，并且在所有报告的情况下，显示出对于病毒生长是必需的，在衣壳成熟和DNA包装中起作用。由于迄今为止仅HSV-1和PrV UL 17蛋白被表征，我们希望检查MDV-1 pUL 17在病毒复制中的作用。为了分析MDV-1 UL 17基因的功能，我们对开放阅读框（ORF）进行了缺失突变或点突变，以中断其编码期。我们确定了功能性ORF UL 17对于MDV-1生长是必不可少的。我们选择通过用与其C-末端融合的HA肽的重复序列标记729个氨基酸长的蛋白质来表征病毒编码的蛋白质。蛋白质pUL 17在感染的细胞提取物中被鉴定为82 kDa的蛋白质，其定位于细胞核，与主要衣壳蛋白VP 5和主要被膜蛋白VP 13/14共定位。通过使用绿色荧光蛋白融合物和在巨细胞病毒IE基因增强子/启动子（PCMV IE）下表达的HA标签蛋白，我们表明MDV-1 pUL 17在感染细胞中的核分布不是固有特性。虽然我们的研究结果强烈表明，另一种病毒蛋白保留（或重新定位）pUL 17到细胞核，我们报告说，没有被膜蛋白测试到目前为止，能够介导pUL 17重新定位到细胞核。
Marek’s disease virus type 1 (MDV-1) shows a strict dependency on the direct cell-to-cell spread for its propagation in cell culture. As MDV-1 shows an impaired nuclear egress in cell culture, we wished to address the characterization of capsid/tegument genes which may intervene in the maturation of intranuclear capsids. Orthologs of UL17 are present in all herpesviruses and, in all reported case, were shown to be essential for viral growth, playing a role in capsid maturation and DNA packaging. As only HSV-1 and PrV UL17 proteins have been characterized so far, we wished to examine the role of MDV-1 pUL17 in virus replication. To analyze MDV-1 UL17 gene function, we created deletion mutants or point mutated the open reading frame (ORF) to interrupt its coding phase. We established that a functional ORF UL17 is indispensable for MDV-1 growth. We chose to characterize the virally encoded protein by tagging the 729 amino-acid long protein with a repeat of the HA peptide that was fused to its C-terminus. Protein pUL17 was identified in infected cell extracts as an 82 kDa protein which localized to the nucleus, colocalizing with VP5, the major capsid protein, and VP13/14, a major tegument protein. By using green fluorescent protein fusion and HA tagged proteins expressed under the cytomegalovirus IE gene enhancer/promoter (PCMV IE), we showed that MDV-1 pUL17 nuclear distribution in infected cells is not an intrinsic property. Although our results strongly suggest that another viral protein retains (or relocate) pUL17 to the nucleus, we report that none of the tegument protein tested so far were able to mediate pUL17 relocation to the nucleus.
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影响因子：
5.4
作者：
COHEN, JI;SEIDEL, K
通讯作者：
SEIDEL, K
影响因子：
5.4
作者：
Mossman, KL;Sherburne, R;Smiley, JR
通讯作者：
Smiley, JR
DOI：
10.1073/pnas.80.16.4949
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
NIMAN, HL;HOUGHTEN, RA;LERNER, RA
通讯作者：
LERNER, RA
影响因子：
5.4
作者：
Petherbridge, L;Brown, AC;Nair, VK
通讯作者：
Nair, VK
影响因子：
5.4
作者：
Schumacher, D;Tischer, BK;Osterrieder, N
通讯作者：
Osterrieder, N