Diverse temperate bacteriophage carriage in Clostridium difficile 027 strains.
Diverse temperate bacteriophage carriage in Clostridium difficile 027 strains.
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DOI：
10.1371/journal.pone.0037263
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Clokie MR
中科院分区：
文献类型：
--
作者：
Nale JY;Shan J;Hickenbotham PT;Fawley WN;Wilcox MH;Clokie MR
高毒力艰难梭菌核糖体型027可分为亚型，但尚不清楚这些亚型在C的严重程度方面是否存在差异。艰难梭菌感染（CDI）。C. difficile 027菌株已经确定它们富含包括原噬菌体在内的移动的遗传元件。本研究结合生理学研究、电子显微镜分析和分子生物学方法，探讨了温性噬菌体在炭疽病中的潜在作用及其多样性。027. honor我们从91株临床C. difficile 027分离物，并使用透射电子显微镜和脉冲场凝胶电泳来鉴定存在的噬菌体。我们建立了噬菌体形态和亚型之间的相关性。在63株和3株分离株中分别鉴定出属于肌尾噬菌体科和虹吸噬菌体科的形态学不同的尾噬菌体。在四个分离株中观察到双噬菌体携带。此外，在所有分离物中均存在可诱导的噬菌体尾样颗粒（PT-LPs）。比较了丝裂霉素C和诺氟沙星两种抗生素对原噬菌体的诱导能力，结果表明，两种抗生素均能诱导特异性的C.艰难分离株。设计了一种针对肌病毒衣壳蛋白基因的PCR方法，以检测C.艰难肌病毒。对8个核糖体型的衣壳蛋白基因序列进行系统发育分析，结果表明，核糖体型027分离株的衣壳蛋白基因序列完全相同，与其它分离株的衣壳蛋白基因序列完全不同。difficile ribotypes和其他细菌物种。一组不同的温和噬菌体与C。027. honor噬菌体携带和亚型之间的相关性表明，温和的噬菌体有助于C。difficile 027，并且可能在与该核糖体型相关的疾病的严重性中起作用。衣壳蛋白基因可作为鉴定C.存在于细菌基因组内的艰难肌病毒。
The hypervirulent Clostridium difficile ribotype 027 can be classified into subtypes, but it unknown if these differ in terms of severity of C. difficile infection (CDI). Genomic studies of C. difficile 027 strains have established that they are rich in mobile genetic elements including prophages. This study combined physiological studies, electron microscopy analysis and molecular biology to determine the potential role of temperate bacteriophages in disease and diversity of C. difficile 027. We induced prophages from 91 clinical C. difficile 027 isolates and used transmission electron microscopy and pulsed-field gel electrophoresis to characterise the bacteriophages present. We established a correlation between phage morphology and subtype. Morphologically distinct tailed bacteriophages belonging to Myoviridae and Siphoviridae were identified in 63 and three isolates, respectively. Dual phage carriage was observed in four isolates. In addition, there were inducible phage tail-like particles (PT-LPs) in all isolates. The capacity of two antibiotics mitomycin C and norfloxacin to induce prophages was compared and it was shown that they induced specific prophages from C. difficile isolates. A PCR assay targeting the capsid gene of the myoviruses was designed to examine molecular diversity of C. difficile myoviruses. Phylogenetic analysis of the capsid gene sequences from eight ribotypes showed that all sequences found in the ribotype 027 isolates were identical and distinct from other C. difficile ribotypes and other bacteria species. A diverse set of temperate bacteriophages are associated with C. difficile 027. The observed correlation between phage carriage and the subtypes suggests that temperate bacteriophages contribute to the diversity of C. difficile 027 and may play a role in severity of disease associated with this ribotype. The capsid gene can be used as a tool to identify C. difficile myoviruses present within bacterial genomes.
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DOI：
10.1073/pnas.58.5.1903
发表时间：
1967-01-01
影响因子：
11.1
作者：
KIRBY, EP;JACOB, F;GOLDTHWAIT, DA
通讯作者：
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影响因子：
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作者：
Horgan, Marianne;O'Sullivan, Orla;Ross, R. Paul
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Ross, R. Paul
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DOI：
10.1073/pnas.0914322107
发表时间：
2010-04-20
影响因子：
11.1
作者：
He, Miao;Sebaihia, Mohammed;Parkhill, Julian
通讯作者：
Parkhill, Julian
影响因子：
3.1
作者：
Lee, FKN;Dudas, KC;Apicella, MA
通讯作者：
Apicella, MA