Acid-sensing ion channel 1a in the central nucleus of the amygdala regulates anxiety-like behaviors in a mouse model of acute pain.
Acid-sensing ion channel 1a in the central nucleus of the amygdala regulates anxiety-like behaviors in a mouse model of acute pain.
复制标题
杏仁核中央核中的酸性离子通道1a在急性疼痛的小鼠模型中调节焦虑样行为。
DOI：
10.3389/fnmol.2022.1006125
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发表时间：
2022
影响因子：
4.8
通讯作者：
Liang, Li-Shuang
中科院分区：
文献类型：
--
作者：
Shi, Pei;Zhang, Ming-Jun;Liu, An;Yang, Chen-Ling;Yue, Jia-Yin;Hu, Rui;Mao, Yu;Zhang, Zhi;Wang, Wei;Jin, Yan;Liang, Li-Shuang
关键词：
疼痛常与焦虑共病；然而，疼痛共病焦虑症状（CASP）背后的神经和分子机制尚未完全阐明。在本研究中，我们探究了位于杏仁核中央核的γ-氨基丁酸（GABA）能神经元（GABACeA）中的酸敏感离子通道1a（ASIC1a）在急性疼痛小鼠模型中对CASP的调节作用。我们发现，在注射完全弗氏佐剂后1天（CFA1D），小鼠表现出明显的机械性痛觉过敏和焦虑样行为。急性脑片的电生理记录显示，与注射生理盐水的小鼠相比，CFA1D小鼠的GABACeA神经元活动增强。此外，化学遗传学方法抑制GABACeA神经元可缓解CFA1D小鼠的机械性痛觉过敏和焦虑样行为。有趣的是，通过药理学抑制和基因敲低杏仁核中央核中的ASIC1a，我们发现ASIC1a的下调减轻了机械刺激的超敏反应，缓解了焦虑相关行为，同时逆转了CFA1D小鼠中GABACeA神经元的过度活动。总之，我们的研究结果提供了新的见解，即GABACeA神经元中的ASIC1a在急性疼痛小鼠模型中调节焦虑样行为。
Pain is commonly comorbid with anxiety; however, the neural and molecular mechanisms underlying the comorbid anxiety symptoms in pain (CASP) have not been fully elucidated. In this study, we explored the role of acid-sensing ion channel 1a (ASIC1a), located in GABAergic neurons from the central nucleus of the amygdala (GABACeA), in the regulation of CASP in an acute pain mouse model. We found that the mice displayed significant mechanical pain sensitization and anxiety-like behaviors one day post injection of complete Freud’s adjuvant (CFA1D). Electrophysiological recordings from acute brain slices showed that the activity of GABACeA neurons increased in the CFA1D mice compared with that in the saline mice. In addition, chemogenetic inhibition of GABACeA neurons relieved mechanical pain sensitization and anxiety-like behaviors in the CFA1D mice. Interestingly, through pharmacological inhibition and genetic knockdown of ASIC1a in the central nucleus amygdala, we found that downregulation of ASIC1a relieved the hypersensitization of mechanical stimuli and alleviated anxiety-related behaviors, accompanied with reversing the hyperactivity of GABACeA neurons in the CFA 1D mice. In conclusion, our results provide novel insights that ASIC1a in GABACeA neurons regulates anxiety-like behaviors in a mouse model of acute pain.
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影响因子：
3.3
作者：
Gonzalez-Inchauspe, Carlota;Natalia Gobetto, Maria;Uchitel, Osvaldo D.
通讯作者：
Uchitel, Osvaldo D.
从杏仁核中的电路到行为。
影响因子：
64.8
作者：
Janak PH;Tye KM
通讯作者：
Tye KM
影响因子：
19.9
作者：
Etkin, Amit;Egner, Tobias;Kalisch, Raffael
通讯作者：
Kalisch, Raffael
影响因子：
15.8
作者：
Gosmann NP;Costa MA;Jaeger MB;Motta LS;Frozi J;Spanemberg L;Manfro GG;Cuijpers P;Pine DS;Salum GA
通讯作者：
Salum GA
DOI：
10.1523/jneurosci.3907-08.2008
发表时间：
2008-12-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Coryell MW;Wunsch AM;Haenfler JM;Allen JE;McBride JL;Davidson BL;Wemmie JA
通讯作者：
Wemmie JA