Gene signatures associated with adaptive humoral immunity following seasonal influenza A/H1N1 vaccination.
Gene signatures associated with adaptive humoral immunity following seasonal influenza A/H1N1 vaccination.
复制标题
DOI：
10.1038/gene.2016.34
复制
发表时间：
2016-12
影响因子：
5
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究旨在鉴定流感血凝抑制（HAI）和病毒中和抗体（VNA）应答之间共有的基因表达标记。我们招募了158名接受2010-2011年三价灭活流感疫苗（TIV）的老年受试者。使用接种疫苗后第0、3和28天获得的血液样本进行流感特异性HAI和VNA滴度以及mrna测序。对于第28天和第0天的抗体反应，几个基因集在HAI （n=7）和VNA （n=35）反应的预测模型中被鉴定为显著。HAI和VNA共有5个基因集（包括基因MAZ、TTF、GSTM、RABGGTA、SMS、CA、IFNG和DOPEY）。对于第28天和第3天的反应，在HAI （n=13）和VNA （n=41）的预测模型中确定了许多基因集。HAI和VNA共有10个基因集（包括生物学相关基因，如MAN1B1、POLL、CEBPG、FOXP3、IL12A、TLR3、TLR7等）。这些确定的基因集显示了高度的网络相互作用和功能关系的可能性。流感特异性HAI和VNA反应表现出显著的相似性。虽然每种体液结果都有独特的基因集特征，但确定有几个基因集与HAI和VNA对流感疫苗的反应相同。
This study aimed to identify gene expression markers shared between both influenza hemagglutination-inhibition (HAI) and virus-neutralization antibody (VNA) responses. We enrolled 158 older subjects who received the 2010–2011 trivalent inactivated influenza vaccine (TIV). Influenza-specific HAI and VNA titers, and mRNA-sequencing were performed using blood samples obtained at Days 0, 3 and 28 post-vaccination. For antibody response at Day 28 vs Day 0, several genesets were identified as significant in predictive models for HAI (n=7) and VNA (n=35) responses. Five genesets (comprising the genes MAZ, TTF, GSTM, RABGGTA, SMS, CA, IFNG, and DOPEY) were in common for both HAI and VNA. For response at Day 28 vs Day 3, many genesets were identified in predictive models for HAI (n=13) and VNA (n=41). Ten genesets (comprising biologically related genes, such as MAN1B1, POLL, CEBPG, FOXP3, IL12A, TLR3, TLR7, and others) were shared between HAI and VNA. These identified genesets demonstrated a high degree of network interactions and likelihood for functional relationships. Influenza-specific HAI and VNA responses demonstrated a remarkable degree of similarity. Although unique geneset signatures were identified for each humoral outcome, several genesets were determined to be in common with both HAI and VNA response to influenza vaccine.
登录
查看更多内容
DOI：
10.1093/biostatistics/kxr054
发表时间：
2012-04
期刊：
Biostatistics (Oxford, England)
影响因子：
--
作者：
Hansen KD;Irizarry RA;Wu Z
通讯作者：
Wu Z
DOI：
10.1128/cdli.5.1.114-117.1998
发表时间：
1998-01-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Benne, CA;Kroon, FP;De Jong, JC
通讯作者：
De Jong, JC
影响因子：
3.7
作者：
Haralambieva IH;Oberg AL;Ovsyannikova IG;Kennedy RB;Grill DE;Middha S;Bot BM;Wang VW;Smith DI;Jacobson RM;Poland GA
通讯作者：
Poland GA
影响因子：
17.1
作者：
Bentebibel SE;Lopez S;Obermoser G;Schmitt N;Mueller C;Harrod C;Flano E;Mejias A;Albrecht RA;Blankenship D;Xu H;Pascual V;Banchereau J;Garcia-Sastre A;Palucka AK;Ramilo O;Ueno H
通讯作者：
Ueno H
影响因子：
5
作者：
FOX, JP;COONEY, MK;FOY, HM
通讯作者：
FOY, HM