Mechanical stress and network structure drive protein dynamics during cytokinesis.
Mechanical stress and network structure drive protein dynamics during cytokinesis.
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DOI：
10.1016/j.cub.2015.01.025
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发表时间：
2015-03-02
期刊：
影响因子：
9.2
通讯作者：
Robinson, Douglas N.
中科院分区：
文献类型：
--
作者：
Srivastava, Vasudha;Robinson, Douglas N.
与胞质分裂和运动等过程相关的细胞形状变化在几秒的时间尺度上进行，但源自分子事件，包括蛋白质-蛋白质相互作用、细丝组装和分子马达产生的力，所有这些都发生得更快。因此，定义这种分子机器的动力学对于理解细胞形状调节至关重要。除了信号通路外，机械应力还直接影响细胞骨架蛋白的积累。基于肌球蛋白 II 的机械感觉系统在胞质分裂期间和施加压力下控制细胞收缩性和形状。在盘基网柄菌属中，该系统通过肌动蛋白网络的反馈调节肌球蛋白 II 的积累，特别是通过交联剂皮质西林 I。皮质西林结合 IQGAP 是该系统的主要调节因子。在这里，我们使用光漂白和荧光相关光谱后的荧光恢复定义了关键细胞骨架蛋白在胞质分裂期间和机械应力下的短时间尺度动态，以检查这些蛋白质之间的动态相互作用。赤道富集的蛋白质（包括皮质西林 I、IQGAP2 和肌球蛋白 II）的恢复速度比肌动蛋白和极性交联剂慢得多。与间期皮层相比，沟道处的赤道蛋白的流动性大大降低，这表明它们在胞质分裂过程中保持稳定。这种迁移率的转变并不是由单一的生化事件引起的，而是由细胞骨架结构中与机械应力相关的变化对蛋白质动力学的整体抑制引起的。收缩蛋白动力学的机械调节为负责调节细胞形状的细胞骨架框架提供了稳健性，并有助于胞质分裂的保真度。
Cell shape changes associated with processes like cytokinesis and motility proceed on several second time-scales, but are derived from molecular events, including protein-protein interactions, filament assembly, and force generation by molecular motors, all of which occur much faster. Therefore, defining the dynamics of such molecular machinery is critical for understanding cell shape regulation. In addition to signaling pathways, mechanical stresses also direct cytoskeletal protein accumulation. A myosin II-based mechanosensory system controls cellular contractility and shape during cytokinesis and under applied stress. In Dictyostelium, this system tunes myosin II accumulation by feedback through the actin network, particularly through the crosslinker cortexillin I. Cortexillin-binding IQGAPs are major regulators of this system. Here, we defined the short time-scale dynamics of key cytoskeletal proteins during cytokinesis and under mechanical stress using fluorescence recovery after photobleaching and fluorescence correlation spectroscopy, to examine the dynamic interplay between these proteins. Equatorially enriched proteins including cortexillin I, IQGAP2, and myosin II recovered much more slowly than actin and polar crosslinkers. The mobility of equatorial proteins was greatly reduced at the furrow compared to the interphase cortex, suggesting their stabilization during cytokinesis. This mobility shift did not arise from a single biochemical event, but rather from a global inhibition of protein dynamics by mechanical stress-associated changes in the cytoskeletal structure. Mechanical tuning of contractile protein dynamics provides robustness to the cytoskeletal framework responsible for regulating cell shape and contributes to cytokinesis fidelity.
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DOI：
10.1083/jcb.200011013
发表时间：
2001-07-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Yumura S
通讯作者：
Yumura S
影响因子：
64.5
作者：
HAUGWITZ, M;NOEGEL, AA;SCHLEICHER, M
通讯作者：
SCHLEICHER, M
影响因子：
21.3
作者：
Murphy, CT;Rock, RS;Spudich, JA
通讯作者：
Spudich, JA
DOI：
10.1073/pnas.0706124105
发表时间：
2008-07-08
影响因子：
11.1
作者：
Ferrer, Jorge M.;Lee, Hyungsuk;Lang, Matthew J.
通讯作者：
Lang, Matthew J.
影响因子：
9.2
作者：
Effler, Janet C.;Kee, Yee-Seir;Robinson, Douglas N.
通讯作者：
Robinson, Douglas N.