Transcriptome profiling of the cold response and signaling pathways in Lilium lancifolium.
Transcriptome profiling of the cold response and signaling pathways in Lilium lancifolium.
复制标题
兰西百合冷反应和信号通路的转录组分析
DOI：
10.1186/1471-2164-15-203
复制
发表时间：
2014-03-17
期刊：
影响因子：
4.4
通讯作者：
Lu Y
中科院分区：
文献类型：
--
作者：
Wang J;Yang Y;Liu X;Huang J;Wang Q;Gu J;Lu Y
背景：百合是一种重要的抗寒野生花卉，广泛分布于中国西南部和东北部，是百合抗寒育种的重要材料。为了更好地了解冷信号通路和参与冷反应的分子代谢反应，我们使用RNA-Seq进行了全基因组转录分析。结果：从3个文库(CK 0h、冷处理2 h和4℃下16 h)获得了约1047.03亿个干净的90bp成对读数；18,736个Uniges与Swiss-Prot蛋白质数据库中的已知蛋白质相似，15,898、13、705和1849个Uniges分别与KEGG和COG数据库中的现有序列(包含25个COG类别)比对，形成了12个SOM簇。基于qRT-PCR结果，我们研究了三种信号调控途径--Ca(2+)和ABA非依赖/依赖途径--将冷信号传导到在鳞茎中高表达的信号转导基因LlICE和LlCDPK，以及转录因子基因LlDREB1/CBF、LlAP2/EREBP、LlNAC1、LlR2R3-MYB和LlBZIP。LlFAD3、L1β-淀粉酶、LlP5CS和LlCLS在低温胁迫下响应冷和增强适应过程，涉及与细胞渗透保护剂和碳水化合物代谢相关的转录本的表达变化。结论：我们对参与冷相关代谢途径和转录因子的差异表达基因的研究，促进了L百合抗寒基因和冷信号转录网络的发现，并确定了参与调控冷反应的潜在关键成分，为进一步研究和深入探索百合抗寒育种候选基因奠定了基础。
Background:Lilium lancifolium, a very important cold-resistant wild flower for lily cold resistance breeding, is widely distributed in southwestern and northeastern China. To gain a better understanding of the cold signaling pathway and the molecular metabolic reactions involved in the cold response, we performed a genome-wide transcriptional analysis using RNA-Seq.Results:Approximately 104,703 million clean 90- bp paired-end reads were obtained from three libraries (CK 0 h, Cold-treated 2 h and 16 h at 4 °C); 18,736 unigenes showed similarity to known proteins in the Swiss-Prot protein database, and 15,898, 13,705 and 1849 unigenes aligned to existing sequences in the KEGG and COG databases (comprising 25 COG categories) and formed 12 SOM clusters, respectively. Based on qRT-PCR results, we studied three signal regulation pathways--the Ca(2+) and ABA independent/dependent pathways--that conduct cold signals to signal transduction genes such as LlICE and LlCDPK and transcription factor genes such as LlDREB1/CBF, LlAP2/EREBP, LlNAC1, LlR2R3-MYB and LlBZIP, which were expressed highly in bulb. LlFAD3, Llβ-amylase, LlP5CS and LlCLS responded to cold and enhanced adaptation processes that involve changes in the expression of transcripts related to cellular osmoprotectants and carbohydrate metabolism during cold stress.Conclusions:Our study of differentially expressed genes involved in cold-related metabolic pathways and transcription factors facilitated the discovery of cold-resistance genes and the cold signal transcriptional networks, and identified potential key components in the regulation of the cold response in L lancifolium, which will be most beneficial for further research and in-depth exploration of cold-resistance breeding candidate genes in lily.
登录
查看更多内容
影响因子：
4.4
作者：
Cheng C;Yun KY;Ressom HW;Mohanty B;Bajic VB;Jia Y;Yun SJ;de los Reyes BG
通讯作者：
de los Reyes BG
影响因子：
6.9
作者：
Ludwig, AA;Romeis, T;Jones, JDG
通讯作者：
Jones, JDG
影响因子：
7.2
作者：
Gilmour, SJ;Zarka, DG;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
56.9
作者：
Moellering, Eric R.;Muthan, Bagyalakshmi;Benning, Christoph
通讯作者：
Benning, Christoph
影响因子：
4
作者：
Qiu, Qiang;Ma, Tao;Liu, Jianquan
通讯作者：
Liu, Jianquan