Spectral-domain optical coherence tomography of the rodent eye: highlighting layers of the outer retina using signal averaging and comparison with histology.
Spectral-domain optical coherence tomography of the rodent eye: highlighting layers of the outer retina using signal averaging and comparison with histology.
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啮齿动物眼的光谱域光学相干断层扫描：使用信号平均和组织学比较，突出显示外视网膜的层。
DOI：
10.1371/journal.pone.0096494
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bemelmans AP
中科院分区：
文献类型：
--
作者：
Berger A;Cavallero S;Dominguez E;Barbe P;Simonutti M;Sahel JA;Sennlaub F;Raoul W;Paques M;Bemelmans AP
光谱域光学相干断层扫描（SD-OCT）是一种广泛应用于观察视网膜各层和跟踪病理事件的方法。最近，这项技术已被用于动物成像。这种非侵入性技术带来了视网膜的横截面可视化，可以精确观察每一层。这种成像方式在啮齿动物中的使用有明显的扩展，因此，现在需要通过SD-OCT观察到的不同视网膜外层的精确表征，以充分利用这项技术。直到外核层的内层的识别已经清楚地建立，而通过SD-OCT观察到的层与感光节段和视网膜色素上皮相对应的结构的归属则更加可疑。为了进一步理解实验SD-OCT成像，我们开发了一种方法，用于平均SD-OCT数据，以生成平均图像，从而更好地描绘色素和白化病品系小鼠和大鼠视网膜中的层。它使我们能够精确地定位光感受器和视网膜色素上皮之间的界面，并明确地识别对应于光感受器节段的内部和外部的四个层。我们表明，在SD-OCT图像上可以准确地测量各个层的厚度，而不是通过组织切片的形态测量分析进行尸检。我们将SD-OCT应用于不同的模型，并证明它可以分析局灶性或弥漫性视网膜病理过程，如光感受器的突变依赖性损伤或光驱动的修饰。此外，我们报告了一种新的方法，结合使用SD-OCT和积分量化激光诱导的脉络膜新生血管。总之，我们清楚地表明，SD-OCT是一种有价值的工具，用于啮齿动物视网膜成像，至少与组织学一样准确，非侵入性，并允许对同一动物进行纵向随访。
Spectral-Domain Optical Coherence Tomography (SD-OCT) is a widely used method to observe retinal layers and follow pathological events in human. Recently, this technique has been adapted for animal imaging. This non-invasive technology brings a cross-sectional visualization of the retina, which permits to observe precisely each layer. There is a clear expansion of the use of this imaging modality in rodents, thus, a precise characterization of the different outer retinal layers observed by SD-OCT is now necessary to make the most of this technology. The identification of the inner strata until the outer nuclear layer has already been clearly established, while the attribution of the layers observed by SD-OCT to the structures corresponding to photoreceptors segments and retinal pigment epithelium is much more questionable. To progress in the understanding of experimental SD-OCT imaging, we developed a method for averaging SD-OCT data to generate a mean image allowing to better delineate layers in the retina of pigmented and albino strains of mice and rats. It allowed us to locate precisely the interface between photoreceptors and retinal pigment epithelium and to identify unambiguously four layers corresponding to the inner and outer parts of photoreceptors segments. We show that the thickness of the various layers can be measured as accurately in vivo on SD-OCT images, than post-mortem by a morphometric analysis of histological sections. We applied SD-OCT to different models and demonstrated that it allows analysis of focal or diffuse retinal pathological processes such as mutation-dependant damages or light-driven modification of photoreceptors. Moreover, we report a new method of combined use of SD-OCT and integration to quantify laser-induced choroidal neovascularization. In conclusion, we clearly demonstrated that SD-OCT represents a valuable tool for imaging the rodent retina that is at least as accurate as histology, non-invasive and allows longitudinal follow-up of the same animal.
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影响因子：
4.4
作者：
Huber G;Beck SC;Grimm C;Sahaboglu-Tekgoz A;Paquet-Durand F;Wenzel A;Humphries P;Redmond TM;Seeliger MW;Fischer MD
通讯作者：
Fischer MD
影响因子：
6.4
作者：
Ferguson LR;Balaiya S;Grover S;Chalam KV
通讯作者：
Chalam KV
影响因子：
1.8
作者：
Paques, M;Sirnonutti, M;Sahel, JA
通讯作者：
Sahel, JA
DOI：
10.1007/978-1-62703-080-9_6
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Grimm, Christian;Reme, Charlotte E
通讯作者：
Reme, Charlotte E
影响因子：
13.7
作者：
Chung, Hyewon;Park, Byeongjun;Kim, Hyung Chan
通讯作者：
Kim, Hyung Chan