Human dendritic cell targeting peptide can be targeted to porcine dendritic cells to improve antigen capture efficiency to stimulate stronger immune response.
Human dendritic cell targeting peptide can be targeted to porcine dendritic cells to improve antigen capture efficiency to stimulate stronger immune response.
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DOI：
10.3389/fimmu.2022.950597
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发表时间：
2022
影响因子：
7.3
通讯作者：
Li, Yijing
中科院分区：
文献类型：
--
作者：
Xia, Tian;Yang, Huizhu;Guo, Yuyao;Guo, Tiantian;Xin, Lingxiang;Jiang, Yanping;Cui, Wen;Zhou, Han;Qiao, Xinyuan;Wang, Xiaona;Li, Jiaxuan;Shan, Zhifu;Tang, Lijie;Wang, Li;Li, Yijing
关键词：
树突状细胞（Dendritic cells，DCs）由于其强大的抗原提呈能力，在病原体的自然识别和随后的适应性免疫应答的激活中起着关键作用。树突状细胞靶向肽（DCpep）是一种强靶向于DC的肽，可增强疫苗的免疫效果。我们前期的研究表明，表达人DCpep的重组乳酸杆菌能显著增强免疫应答，但其机制尚不清楚。在这项研究中，DCpep增强对重组乳酸杆菌的免疫应答的机制进行了探索。合成了荧光标记的人DCpep，以评价人DCpep与猪单核细胞来源的树突状细胞（Mo-DCs）和小肠DCs的结合能力。研究了表达人DCpep融合猪流行性腹泻病毒（PEDV）核心中和表位（COE）抗原的重组乳杆菌对Mo-DC功能的影响。结果表明，人DCpep与猪DC结合，但不与猪小肠上皮细胞结合。人DCpep还可以提高Mo-DCs对重组乳酸杆菌的捕获效率，促进树突状细胞成熟，分泌更多的细胞因子，增强猪DCs激活T细胞增殖的能力。总之，这些结果促进了对DCpep增强免疫反应机制的深入理解。我们发现人和猪之间的某些DCpep是保守的，这为DC靶向疫苗的开发提供了理论基础。
Dendritic cells (DCs) play a key role in the natural recognition of pathogens and subsequent activation of adaptive immune responses due to their potent antigen-presenting ability. Dendritic cell-targeting peptide (DCpep) is strongly targeted to DCs, which often express antigens, to enhance the efficacy of vaccines. Our previous study showed that recombinant Lactobacillus expressing human DCpep could significantly induce stronger immune responses than recombinant Lactobacillus without DCpep, but the mechanism remains unclear. In this study, the mechanism by which DCpep enhances the immune response against recombinant Lactobacillus was explored. Fluorescence-labeled human DCpep was synthesized to evaluate the binding ability of human DCpep to porcine monocyte-derived dendritic cells (Mo-DCs) and DCs of the small intestine. The effects of Mo-DC function induced by recombinant Lactobacillus expressing human DCpep fused with the porcine epidemic diarrhea virus (PEDV) core neutralizing epitope (COE) antigen were also investigated. The results showed that human DCpep bind to porcine DCs, but not to porcine small intestinal epithelial cells. Human DCpep can also improve the capture efficiency of recombinant Lactobacillus by Mo-DCs, promote the maturation of dendritic cells, secrete more cytokines, and enhance the ability of porcine DCs to activate T-cell proliferation. Taken together, these results promote advanced understanding of the mechanism by which DCpep enhances immune responses. We found that some DCpeps are conserved between humans and pigs, which provides a theoretical basis for the development of a DC-targeted vaccine.
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