A unique form of collective epithelial migration is crucial for tissue fusion in the secondary palate and can overcome loss of epithelial apoptosis.
A unique form of collective epithelial migration is crucial for tissue fusion in the secondary palate and can overcome loss of epithelial apoptosis.
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一种独特形式的集体上皮迁移是至关重要的组织融合在继发腭，可以克服上皮细胞凋亡的损失。
DOI：
10.1242/dev.200181
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发表时间：
2022-05-15
期刊：
影响因子：
--
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中科院分区：
文献类型：
--
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组织融合通常需要去除上皮，该上皮介入不同的原基以形成一个连续的结构。在哺乳动物的第二腭，中线上皮缝（MES）之间形成两个腭架，必须删除，以允许间充质融合。大量的凋亡和细胞挤出支持它们在MES去除中的重要性。然而，遗传破坏MES内的内在凋亡调节因子BAX和BAK导致细胞死亡和细胞挤出的完全丧失，但成功去除MES。新的静态和实时成像方法显示，MES是通过流动迁移的上皮线索和岛屿，以达到口腔和鼻腔上皮表面。表达基础上皮标记物Δ Np63的上皮trail细胞开始表达周向标记物，表明迁移伴随着分化。实时成像显示上皮内的各向异性肌动球蛋白收缩性，肌动球蛋白收缩性的基因消融导致上皮集合的分散和正常MES迁移的失败。这些研究结果表明，冗余的细胞机制的形态发生，并揭示了一个重要的和独特的形式集体上皮细胞迁移组织融合过程中。总结：多个细胞过程介导继发性腭融合，包括一种独特的形式的流动集体上皮迁移驱动的脉动肌动球蛋白收缩。
Tissue fusion frequently requires the removal of an epithelium that intervenes distinct primordia to form one continuous structure. In the mammalian secondary palate, a midline epithelial seam (MES) forms between two palatal shelves and must be removed to allow mesenchymal confluence. Abundant apoptosis and cell extrusion support their importance in MES removal. However, genetically disrupting the intrinsic apoptotic regulators BAX and BAK within the MES results in complete loss of cell death and cell extrusion, but successful removal of the MES. Novel static- and live-imaging approaches reveal that the MES is removed through streaming migration of epithelial trails and islands to reach the oral and nasal epithelial surfaces. Epithelial trail cells that express the basal epithelial marker ΔNp63 begin to express periderm markers, suggesting that migration is concomitant with differentiation. Live imaging reveals anisotropic actomyosin contractility within epithelial trails, and genetic ablation of actomyosin contractility results in dispersion of epithelial collectives and failure of normal MES migration. These findings demonstrate redundancy between cellular mechanisms of morphogenesis, and reveal a crucial and unique form of collective epithelial migration during tissue fusion. Summary: Multiple cellular processes mediate secondary palate fusion, including a unique form of streaming collective epithelial migration driven by pulsatile actomyosin contractility.
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影响因子：
4.6
作者：
Cuervo, R;Covarrubias, L
通讯作者：
Covarrubias, L
影响因子：
3.3
作者：
Blavier, L;Lazaryev, A;Kaartinen, V
通讯作者：
Kaartinen, V
影响因子：
2.7
作者：
Charoenchaikorn, Kesinee;Yokomizo, Tomomasa;Yamashiro, Takashi
通讯作者：
Yamashiro, Takashi
影响因子：
4
作者：
Yamamoto S;Kurosaka H;Miura J;Aoyama G;Sarper SE;Oka A;Inubushi T;Nakatsugawa K;Usami Y;Toyosawa S;Yamashiro T
通讯作者：
Yamashiro T
DOI：
10.1016/j.jgg.2016.04.008
发表时间：
2016-05-20
期刊：
Journal of genetics and genomics = Yi chuan xue bao
影响因子：
--
作者：
Haeussler M;Concordet JP
通讯作者：
Concordet JP