Hypochlorous acid inactivates oral pathogens and a SARS-CoV-2-surrogate.
Hypochlorous acid inactivates oral pathogens and a SARS-CoV-2-surrogate.
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DOI：
10.1186/s12903-023-02820-7
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发表时间：
2023-02-18
期刊：
影响因子：
2.9
通讯作者：
Sasaki, Hajime
中科院分区：
文献类型：
--
作者：
Tazawa, Kento;Jadhav, Rutuja;Azuma, Mariane Maffei;Fenno, J. Christopher;McDonald, Neville J.;Sasaki, Hajime
关键词：
牙科手术过程中产生的飞沫和气溶胶是微生物和病毒传播的危险因素。与次氯酸钠不同，次氯酸（HOCl）对组织无毒，但仍具有广泛的杀微生物作用。HOCl溶液可作为水和/或漱口水的补充。本研究旨在结合口腔诊疗环境，评价HOCl溶液对人类口腔常见病原体及SARS-CoV-2代药MHV A59病毒的防治效果。用3%盐酸电解生成HOCl。从四个方面研究了HOCl对人口腔致病菌核梭菌、中间普雷沃氏菌、中间链球菌、微小小单胞菌和MHV A59病毒的影响：浓度；体积;唾液的存在；和存储。利用不同条件下的HOCl溶液进行杀菌和杀病毒试验，确定完全抑制病原菌所需的最小抑菌体积比。在没有唾液的情况下，新鲜制备的HOCl溶液（45-60 ppm）对细菌悬浮液的最小抑制体积比为4:1，对病毒悬浮液的最小抑制体积比为6:1。唾液的存在使细菌和病毒的最小抑制体积比分别增加到8:1和7:1。使用较高浓度的HOCl溶液（220或330 ppm）不会导致对中间葡萄球菌和微芽孢杆菌的最小抑制体积比显著降低。最小抑制体积比增加的应用HOCl溶液通过牙科单位水管。HOCl溶液贮存1周后，HOCl降解，最小生长抑制体积比增加。HOCl溶液（45-60 ppm）即使在唾液存在和通过牙科单位水线后，仍然对口腔病原体和sars - cov -2替代病毒有效。这项研究表明，HOCl溶液可以用作治疗用水或漱口水，并可能最终降低牙科实践中空气传播感染的风险。
Droplets and aerosols produced during dental procedures are a risk factor for microbial and viral transmission. Unlike sodium hypochlorite, hypochlorous acid (HOCl) is nontoxic to tissues but still exhibits broad microbicidal effect. HOCl solution may be applicable as a supplement to water and/or mouthwash. This study aims to evaluate the effectiveness of HOCl solution on common human oral pathogens and a SARS-CoV-2 surrogate MHV A59 virus, considering the dental practice environment. HOCl was generated by electrolysis of 3% hydrochloric acid. The effect of HOCl on human oral pathogens, Fusobacterium nucleatum, Prevotella intermedia, Streptococcus intermedius, Parvimonas micra, and MHV A59 virus was studied from four perspectives: concentration; volume; presence of saliva; and storage. HOCl solution in different conditions was utilized in bactericidal and virucidal assays, and the minimum inhibitory volume ratio that is required to completely inhibit the pathogens was determined. In the absence of saliva, the minimum inhibitory volume ratio of freshly prepared HOCl solution (45–60 ppm) was 4:1 for bacterial suspensions and 6:1 for viral suspensions. The presence of saliva increased the minimum inhibitory volume ratio to 8:1 and 7:1 for bacteria and viruses, respectively. Applying a higher concentration of HOCl solution (220 or 330 ppm) did not lead to a significant decrease in the minimum inhibitory volume ratio against S. intermedius and P. micra. The minimum inhibitory volume ratio increases in applications of HOCl solution via the dental unit water line. One week of storage of HOCl solution degraded HOCl and increased the minimum growth inhibition volume ratio. HOCl solution (45–60 ppm) is still effective against oral pathogens and SAR-CoV-2 surrogate viruses even in the presence of saliva and after passing through the dental unit water line. This study indicates that the HOCl solution can be used as therapeutic water or mouthwash and may ultimately reduce the risk of airborne infection in dental practice.
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DOI：
10.1292/jvms.14-0413
发表时间：
2015-02
期刊：
The Journal of veterinary medical science
影响因子：
--
作者：
Hakim H;Thammakarn C;Suguro A;Ishida Y;Kawamura A;Tamura M;Satoh K;Tsujimura M;Hasegawa T;Takehara K
通讯作者：
Takehara K
影响因子：
--
作者：
Leibowitz, Julian;Kaufman, Gili;Liu, Pinghua
通讯作者：
Liu, Pinghua
影响因子：
4.1
作者：
Hawkins, CL;Davies, MJ
通讯作者：
Davies, MJ
影响因子：
2.4
作者：
Lafaurie, G. I.;Zaror, C.;Calderon-Mendoza, J.
通讯作者：
Calderon-Mendoza, J.
影响因子：
7.6
作者：
LAGERLOF, F;DAWES, C
通讯作者：
DAWES, C