Evaluation of sprayed hypochlorous acid solutions for their virucidal activity against avian influenza virus through in vitro experiments.
Evaluation of sprayed hypochlorous acid solutions for their virucidal activity against avian influenza virus through in vitro experiments.
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DOI：
10.1292/jvms.14-0413
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Takehara K
中科院分区：
文献类型：
--
作者：
Hakim H;Thammakarn C;Suguro A;Ishida Y;Kawamura A;Tamura M;Satoh K;Tsujimura M;Hasegawa T;Takehara K
评价了次氯酸（HOCl）溶液对低致病性禽流感病毒（AIV）H7 N1的杀病毒能力。将含有50、100和200 ppm氯的HOCl溶液（pH 6）或它们的喷雾溶液（收获在置于喷嘴和皿之间1或30 cm距离处的皿中）与病毒混合，加入或不加入有机材料（5%胎牛血清：FBS）。在普通稀释剂条件下（不含FBS），喷洒后收获的HOCl溶液可使AIV滴度降低1，000倍以上，在5秒内降至无法检测的水平（< 2.5 log 10 TCID 50/ml），但在30 cm距离喷洒后收获的50 ppm溶液除外。在肮脏的条件下（在5%FBS的存在下），它们失去了它们的杀病毒活性。当HOCl溶液直接喷洒在人造丝片上的病毒上10秒时，100和200 ppm的溶液在喷洒后立即就能杀灭AIV，而50 ppm的溶液需要至少3分钟的接触时间。在间接喷雾形式中，在喷雾10秒后，打开培养皿的盖子以将人造丝片材上的病毒暴露于HOCl。在这种形式下，200 ppm的溶液在接触10分钟内灭活AIV，而50和100 ppm不能杀灭它。这些数据表明，HOCl可以以喷雾形式在农场水平上杀灭AIV。
Hypochlorous acid (HOCl) solutions were evaluated for their virucidal ability against a low pathogenic avian influenza virus (AIV), H7N1. HOCl solutions containing 50, 100 and 200 ppm chlorine (pH 6) or their sprayed solutions (harvested in dishes placed at 1 or 30 cm distance between the spray nozzle and dish) were mixed with the virus with or without organic materials (5% fetal bovine serum: FBS). Under plain diluent conditions (without FBS), harvested solutions of HOCl after spraying could decrease the AIV titer by more than 1,000 times, to an undetectable level (< 2.5 log10TCID50/ml) within 5 sec, with the exception of the 50 ppm solution harvested after spraying at the distance of 30 cm. Under the dirty conditions (in the presence of 5% FBS), they lost their virucidal activity. When HOCl solutions were sprayed directly on the virus on rayon sheets for 10 sec, the solutions of 100 and 200 ppm could inactivate AIV immediately after spraying, while 50 ppm solution required at least 3 min of contact time. In the indirect spray form, after 10 sec of spraying, the lids of the dishes were opened to expose the virus on rayon sheets to HOCl. In this form, the 200 ppm solution inactivated AIV within 10 min of contact, while 50 and 100 ppm could not inactivate it. These data suggest that HOCl can be used in spray form to inactivate AIV at the farm level.
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