PACAP expression in explant cultured mouse major pelvic ganglia.
PACAP expression in explant cultured mouse major pelvic ganglia.
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DOI：
10.1007/s12031-010-9359-4
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发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Parsons RL
中科院分区：
文献类型：
--
作者：
Girard BM;Galli JR;Young BA;Vizzard MA;Parsons RL
大盆腔神经节（MPG）包含副交感神经和交感神经节后神经元，并为泌尿生殖器官和下肠组成部分提供许多自主神经支配。虽然许多副交感神经细胞表达血管活性肠多肽（VIP），但MPG神经元未表现出垂体腺苷酸环化酶激活多肽（PACAP）的免疫反应性。然而，在培养3天的MPG中，大量的PACAP- ir细胞和神经纤维存在，并且PACAP转录水平显著增加，因为MPG中含有交感和副交感神经节后神经元。在培养3 d的MPG中，在VIP或神经元一氧化氮合酶（nNOS）免疫反应的细胞中可以观察到PACAP的免疫反应，但在酪氨酸羟化酶（TH）免疫反应的细胞中没有，这表明PACAP的表达优先发生在MPG副交感神经节后神经元中。在培养3天后，VPAC2的转录水平也显著增加，而VPAC1或PAC1受体的转录水平则没有显著增加。激活转录因子3 （ATF-3）的转录水平在3天的外植体中增加了64倍，ATF-3- ir核在TH-IR和nNOS-IR神经元以及非神经元细胞中都很明显。综上所述，虽然外植体培养的MPG中只有副交感神经细胞增加了PACAP的表达，但培养的MPG整体中交感和副交感神经节后神经元均增加了ATF-3的表达。
The major pelvic ganglia (MPG) contain both parasympathetic and sympathetic postganglionic neurons and provide much of the autonomic innervation to urogenital organs and components of the lower bowel. Whereas many parasympathetic neurons were found to express vasoactive intestinal polypeptide (VIP), no MPG neurons exhibited immunoreactivity for pituitary adenylate cyclase activating polypeptide (PACAP). However, in 3-day cultured MPGs, numerous PACAP-IR cells and nerve fibers were present and transcript levels for PACAP increase As the MPG contains both sympathetic and parasympathetic postganglionic neurons, d significantly. In 3-day cultured MPGs, PACAP immunoreactivity was seen in cells that were also immunoreactive for VIP or neuronal nitric oxide synthase (nNOS), but not tyrosine hydroxylase (TH), indicating that PACAP expression occurred preferentially in MPG parasympathetic postganglionic neurons. Transcript levels for the VPAC2, but not VPAC1 or PAC1 receptor, also increased significantly following 3 days in culture. Transcript levels of activating transcription factor 3 (ATF-3), a marker of cellular injury, were increased 64-fold in 3-day explants and ATF-3-IR nuclei were evident in both TH-IR and nNOS-IR neurons as well as in non-neuronal cells. In sum, these results demonstrate that although only the parasympathetic neurons in explant cultured MPGs increase expression of PACAP, both sympathetic and parasympathetic postganglionic neurons in the cultured MPG whole mount increase expression of ATF-3.
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影响因子：
4.7
作者：
Girard, Beatrice A.;Lelievre, Vincent;May, Victor
通讯作者：
May, Victor
影响因子：
3.1
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3.4
作者：
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3.6
作者：
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