Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe
Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe
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通过近红外荧光探针对凝胶和活细胞中的 G-四链体进行可视化
DOI：
10.1016/j.snb.2016.05.162
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Zhou Xiang
中科院分区：
文献类型：
--
作者：
Wu Fan;Liu Chaoxing;Chen Yuqi;Yang Shixi;Xu Jiahui;Huang Rong;Wang Xiang;Li Manjia;Liu Wenting;Mao Wuxiang;Zhou Xiang
G-四链体是由G四联体堆积而成的一类重要的核酸二级结构，由于其参与端粒功能、基因转录和重组而受到广泛关注。对于配体，G-四链体对其他DNA或RNA结构的更好的选择性和更高的稳定G-四链体的能力是必要的。此外，开发一种新的识别G-四链体的探针，将G-四链体结构应用于相关的生物学过程也是一个迫切需要解决的问题。在这项研究中，我们报告了Cy 5标记的配体（命名为PDP-Cy 5）开发作为近红外荧光配体的G-四链体在DNA和RNA。结果表明，PDP-Cy 5选择性地诱导形成具有强结合亲和力的分子内G-四链体。此外，这种Cy 5标记的配体可以有效地稳定G-四链体。此外，G-四链体在凝胶中的直接可视化，甚至在细胞水平上是通过使用PDP-Cy 5实现的。
G-quadruplexes, one of the most significant secondary structure of nucleic acid, are formed by stacking G quartets, which received broad interests due to their involvement in telomere function, gene transcription and recombination. As for ligand, better selectivity for G-quadruplex against other DNA or RNA structures and higher ability to stabilize G-quadruplex are necessary. In addition, developing a new probe to recognize G-quadruplex is desired for utilizing G-quadruplex structure to relevant biological processes. In this study, we report a Cy5 labelled ligand (named PDP-Cy5) developed as a near-infrared fluorescent ligand for G-quadruplexes both in DNA and RNA. The results indicated that PDP-Cy5 selectively induced the formation of intramolecular G-quadruplexes with strong binding affinity. Furthermore, this Cy5 labelled ligand can effectively stabilize G-quadruplexes. Moreover, the direct visualization of G-quadruplexes in gel, even on cell level is realized by using PDP-Cy5.
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影响因子：
14.9
作者：
Bugaut A;Balasubramanian S
通讯作者：
Balasubramanian S
影响因子：
15
作者：
He, F;Tang, YL;Zhu, DB
通讯作者：
Zhu, DB
影响因子：
11.4
作者：
Schaeffer, C;Bardoni, B;Moine, H
通讯作者：
Moine, H
影响因子：
2.1
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A. Martí;Xiaoxu Li;S. Jockusch;Nathan Stevens;Zengmin Li;B. Raveendra;S. Kalachikov;I. Morozova;J. Russo;D. Akins;J. Ju;N. Turro
影响因子：
3.2
作者：
Masayuki Tera;Keisuke Iida;K. Ikebukuro;H. Seimiya;K. Shin‐ya;K. Nagasawa
通讯作者：
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