The identification of new candidate genes Triticum aestivum FLOWERING LOCUS T3-B1 (TaFT3-B1) and TARGET OF EAT1 (TaTOE1-B1) controlling the short-day photoperiod response in bread wheat.
The identification of new candidate genes Triticum aestivum FLOWERING LOCUS T3-B1 (TaFT3-B1) and TARGET OF EAT1 (TaTOE1-B1) controlling the short-day photoperiod response in bread wheat.
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DOI：
10.1111/pce.13018
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Griffiths S
中科院分区：
文献类型：
--
作者：
Zikhali M;Wingen LU;Leverington-Waite M;Specel S;Griffiths S
对光周期变化的感知使植物能够在最佳的生存条件下开花。我们使用 Avalon × Cadenza、Charger × Badger 和 Spark × Rialto 杂交的双单倍体群体，并在小麦染色体 1BS 和 1BL 上鉴定了短日照开花时间响应数量性状位点 (QTL)。我们利用二穗短柄草和小麦之间的同线性来鉴定这两个 QTL 的潜在候选者。 1BL QTL 峰与 TaFT3-B1 重合，TaFT3-B1 是大麦基因 HvFT3 的同源物，是最有可能的候选基因。 1BS QTL 峰与拟南芥 S ENSITIVITY TO红光降低 1、WUSCHEL-like 和 RAP2.7 的同源物重合，也称为玉米 TARGET OF EAT1，分别命名为 TaSRR1-B1、TaWUSCHELL-B1 和 TaTOE1-B1。基因表达测定表明 TaTOE1-B1 和 TaFT3-B1 在短日内表达较多。我们鉴定了 TaFT3-B1 的四个等位基因和 TaTOE1-B1 的三个等位基因。我们分别使用 936 和 431 种质研究了这些等位基因在 Watkins 和 GEDIFLUX 多样性组中的影响。 TaFT3-B1 缺失导致的损失与晚花有关。 TaFT3-B1 拷贝数增加与提前开花相关，表明 TaFT3-B1 促进开花。在 GEDIFLUX 收集中观察到 TaTOE1-B1（一种假定的开花抑制因子）的显着关联。我们鉴定了两个新的短光周期小麦开花时间基因 TaFT3-B1 和 TaTOE1-B1，分别促进和抑制开花。我们表明，大麦中 Ppd-H2 的同源物 TaFT3-B1 和 TaTOE-B1（谷物研究较少）可能是我们报告的短日照特异性 QTL 的候选者。拟南芥中的 TaTOE1-B1 同源物参与衰老途径，表明小麦和可能的其他谷物也使用相同的途径。我们为这些基因鉴定的 TaFT3-B1 的四个等位基因和 TaTOE1-B1 的三个等位基因为小麦开花时间控制的微调提供了替代途径。
Perception of photoperiod changes enables plants to flower under optimum conditions for survival. We used doubled haploid populations of crosses among Avalon × Cadenza, Charger × Badger and Spark × Rialto and identified short‐day flowering time response quantitative trait loci (QTL) on wheat chromosomes 1BS and 1BL. We used synteny between Brachypodium distachyon and wheat to identify potential candidates for both QTL. The 1BL QTL peak coincided with TaFT3‐B1, a homologue of the barley gene HvFT3, the most likely candidate gene. The 1BS QTL peak coincided with homologues of Arabidopsis thaliana S ENSITIVITY TO R ED LIGHT R EDUCED 1, WUSCHEL‐like and RAP2.7, which is also known as Zea mays TARGET OF EAT1, named TaSRR1‐B1, TaWUSCHELL‐B1 and TaTOE1‐B1, respectively. Gene expression assays suggest that TaTOE1‐B1 and TaFT3‐B1 are expressed more during short days. We identified four alleles of TaFT3‐B1 and three alleles of TaTOE1‐B1. We studied the effect of these alleles in the Watkins and GEDIFLUX diversity panels by using 936 and 431 accessions, respectively. Loss of TaFT3‐B1 by deletion was associated with late flowering. Increased TaFT3‐B1 copy number was associated with early flowering, suggesting that TaFT3‐B1 promotes flowering. Significant association was observed in the GEDIFLUX collection for TaTOE1‐B1, a putative flowering repressor. We identify two novel short photoperiod wheat flowering time genes TaFT3‐B1 and TaTOE1‐B1 that promote and repress flowering, respectively. We show that TaFT3‐B1, the homologue of Ppd‐H2 in barley, and TaTOE‐B1 (less studies in the cereals) are likely candidates for the short‐day‐specific QTL that we report upon. The TaTOE1‐B1 homologue in Arabidopsis thaliana is involved in the ageing pathway, suggesting that wheat and possibly other cereals use the same pathway. The four alleles of TaFT3‐B1 and three alleles of TaTOE1‐B1 we identified for these genes present alternative routes for the fine tuning of flowering time control in wheat.
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影响因子：
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