Role of inflammatory related gene expression in clear cell renal cell carcinoma development and clinical outcomes.
Role of inflammatory related gene expression in clear cell renal cell carcinoma development and clinical outcomes.
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DOI：
10.1016/j.juro.2011.06.049
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发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
Wu X
中科院分区：
文献类型：
--
作者：
Tan W;Hildebrandt MA;Pu X;Huang M;Lin J;Matin SF;Tamboli P;Wood CG;Wu X
肾细胞癌是美国第八大最常见的癌症，透明细胞肾癌是最常见的类型。许多信号通路涉及透明细胞肾癌的发展，包括炎症通路。然而，对于这一途径中的基因表达变异如何影响透明细胞肾癌的发展和临床结果，我们知之甚少。使用全基因组表达阵列检测93例患者肿瘤及邻近正常组织中的基因表达。然后分析了661个炎症相关基因。发现肿瘤组织与正常组织的差异表达模式。使用独立的平台（实时定量聚合酶链反应）在258个肿瘤的验证集中进一步测试了显示显著相关性的基因，以评估与复发或生存的关联。我们鉴定出151个基因在邻近正常组织和肿瘤之间至少有两倍的基因表达变化，其中大多数在肿瘤中上调。共选择20个与复发和/或总生存显著相关的基因进行进一步验证。在复制数据集中，GADD45G高表达与2.09倍（95% CI 1.08 ~ 6.14, p = 0.034）复发风险增加显著相关，而CARD9、NCF2和CIITA高表达分别与2.52倍（95% CI 1.24 ~ 5.12, p = 0.010）、2.26倍（95% CI 1.12 ~ 4.58, p = 0.023）和2.11倍（95% CI 1.05 ~ 4.27, p = 0.037）死亡风险增加显著相关。结果提示，炎症基因表达可能是透明细胞肾癌患者复发风险（GADD45G）和死亡风险（CARD9、CIITA和NCF2）的重要预后生物标志物。
Renal cell carcinoma is the eighth most common cancer in the United States and clear cell renal carcinoma is the most common type. Many signaling pathways are implicated in clear cell renal carcinoma development, including the inflammation pathway. However, less is known about how gene expression variation in this pathway influences clear cell renal carcinoma development and clinical outcomes. Gene expression in tumor and adjacent normal tissues from 93 patients was detected using a genome-wide expression array. A panel of 661 inflammation related genes was then analyzed. Differential expression patterns between tumor and normal tissues were identified. Association with recurrence or survival was evaluated with genes showing significant association tested further in a validation set of 258 tumors using an independent platform (quantitative real-time polymerase chain reaction). We identified 151 genes with at least a two-fold change in gene expression between adjacent normal tissue and tumor, of which most were up-regulated in tumors. A total of 20 genes significantly associated with recurrence and/or overall survival were selected for further validation. In the replication data set high expression of GADD45G was significantly associated with a 2.09-fold (95% CI 1.08 – 6.14, p = 0.034) increased risk of recurrence while high CARD9, NCF2 and CIITA expression was significantly associated with a 2.52-fold (95% CI 1.24 –5.12, p = 0.010), 2.26-fold (95% CI 1.12– 4.58, p = 0.023) and 2.11-fold (95% CI 1.05– 4.27, p = 0.037) increased risk of death, respectively. Results suggest that inflammation gene expression may be significant prognostic biomarkers for the risk of recurrence (GADD45G) and death (CARD9, CIITA and NCF2) in patients with clear cell renal carcinoma.
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影响因子：
254.7
作者：
Jemal, Ahmedin;Siegel, Rebecca;Thun, Michael J.
通讯作者：
Thun, Michael J.
影响因子：
3.9
作者：
Engers, R.;Ziegler, S.;Gabbert, H. E.
通讯作者：
Gabbert, H. E.
影响因子：
56.9
作者：
LETO, TL;LOMAX, KJ;MALECH, HL
通讯作者：
MALECH, HL
与炎症相关的基因组装，用于途径为中心的遗传分析。
影响因子：
3.7
作者：
Loza, Matthew J.;McCall, Charles E.;Li, Liwu;Isaacs, William B.;Xu, Jianfeng;Chang, Bao-Li
通讯作者：
Chang, Bao-Li
DOI：
10.1186/1756-9966-27-53
发表时间：
2008-10-19
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Meteoglu I;Erdogdu IH;Meydan N;Erkus M;Barutca S
通讯作者：
Barutca S