The metagenomic data life-cycle: standards and best practices.
The metagenomic data life-cycle: standards and best practices.
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DOI:
10.1093/gigascience/gix047
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发表时间:
2017-08-01
期刊:
影响因子:
9.2
通讯作者:
Cochrane G
中科院分区:
文献类型:
--
作者:
Ten Hoopen P;Finn RD;Bongo LA;Corre E;Fosso B;Meyer F;Mitchell A;Pelletier E;Pesole G;Santamaria M;Willassen NP;Cochrane G
Metagenomics data analyses from independent studies can only be compared if the analysis workflows are described in a harmonized way. In this overview, we have mapped the landscape of data standards available for the description of essential steps in metagenomics: (i) material sampling, (ii) material sequencing, (iii) data analysis, and (iv) data archiving and publishing. Taking examples from marine research, we summarize essential variables used to describe material sampling processes and sequencing procedures in a metagenomics experiment. These aspects of metagenomics dataset generation have been to some extent addressed by the scientific community, but greater awareness and adoption is still needed. We emphasize the lack of standards relating to reporting how metagenomics datasets are analysed and how the metagenomics data analysis outputs should be archived and published. We propose best practice as a foundation for a community standard to enable reproducibility and better sharing of metagenomics datasets, leading ultimately to greater metagenomics data reuse and repurposing.
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影响因子:
14.9
作者:
Cochrane G;Karsch-Mizrachi I;Takagi T;International Nucleotide Sequence Database Collaboration
通讯作者:
International Nucleotide Sequence Database Collaboration
影响因子:
--
作者:
Huntemann M;Ivanova NN;Mavromatis K;Tripp HJ;Paez-Espino D;Tennessen K;Palaniappan K;Szeto E;Pillay M;Chen IM;Pati A;Nielsen T;Markowitz VM;Kyrpides NC
通讯作者:
Kyrpides NC
影响因子:
14.9
作者:
Mitchell A;Bucchini F;Cochrane G;Denise H;ten Hoopen P;Fraser M;Pesseat S;Potter S;Scheremetjew M;Sterk P;Finn RD
通讯作者:
Finn RD
影响因子:
3
作者:
Meyer, F.;Paarmann, D.;Edwards, R. A.
通讯作者:
Edwards, R. A.
影响因子:
3.4
作者:
Orchard, S;Hermjakob, H;Apweiler, R
通讯作者:
Apweiler, R