Radical pair based magnetic field effects in cells: the importance of photoexcitation conditions and single cell measurements

Radical pair based magnetic field effects in cells: the importance of photoexcitation conditions and single cell measurements
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细胞中基于自由基对的磁场效应:光激发条件和单细胞测量的重要性

DOI:
10.1101/2022.11.09.515724
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发表时间:
2022
期刊:
biorXiv
影响因子:
--
通讯作者:
Noboru Ikeya
Noboru Ikeya
中科院分区:
--
文献类型:
--
作者:
Jonathan R. Woodward;Noboru Ikeya

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bioRxiv 预印本服务器上最近发表的一篇文章旨在复制我们对磁场对 HeLa 细胞自发荧光影响的观察,但无法重现我们原始工作中描述的效果。在这里,我们检查了这项新研究,并基于反应光循环模型证明了为什么所使用的测量条件的差异导致实验不太可能观察到最初报告的效果。此外,我们强调了重复研究中数据质量的重大问题,并重申了原始工作中提出的直接调制、单细胞测量方法相对于更标准的统计方法的优势。
A recent publication on the bioRxiv preprint server aims to replicate our observation of magnetic field effects on the autofluorescence of HeLa cells, but is unable to reproduce the effects described in our original work. Here we examine this new study and demonstrate, based on a model of the reaction photocycle, why the differences in the measurement conditions used render the experiment unlikely to be able to observe the originally reported effect. In addition, we highlight substantial problems in the quality of the data in the replicate study and reiterate the advantages of the direct modulation, single cell measurement approach presented in the original work over a more standard statistical approach.
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