Lactobacillus spp. attenuate antibiotic-induced immune and microbiota dysregulation in honey bees.
Lactobacillus spp. attenuate antibiotic-induced immune and microbiota dysregulation in honey bees.
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DOI：
10.1038/s42003-020-01259-8
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发表时间：
2020-09-25
影响因子：
5.9
通讯作者：
Reid G
中科院分区：
文献类型：
--
作者：
Daisley BA;Pitek AP;Chmiel JA;Gibbons S;Chernyshova AM;Al KF;Faragalla KM;Burton JP;Thompson GJ;Reid G
抗生素在养蜂业中的广泛使用极大地促进了抗生素耐药性的全球传播，并有可能对蜜蜂（Apis mellifera）的细菌共生体产生负面影响。在这里，我们表明，常规抗生素与土霉素的管理选择性地增加tetB（外排泵抗性基因）丰度在肠道菌群的成年工人，同时耗尽几个关键的共生体已知调节免疫功能和营养代谢，如Frischella perrera和乳杆菌坚定-5菌株。这些微生物变化的功能特点是减少封顶育雏计数（标志蜂巢营养状况和生产力）和降低成人血淋巴的抗菌能力（免疫能力的指标）。重要的是，使用三种免疫刺激性乳杆菌菌株的联合治疗可以减轻成年工人中与益生菌相关的微生物群生态失调和免疫缺陷，并通过将幼虫病原体负荷抑制到接近不可检测的水平来最大化土霉素的预期益处。我们的结论是，基于微生物的治疗方法可能提供一个简单而有效的解决方案，以减少蜜蜂疾病负担，环境外源性暴露，和抗菌素耐药性的传播。Daisley等人表明，用土霉素进行抗生素治疗会损害蜜蜂的肠道微生物群和免疫系统，并减少封顶的幼蜂数量。他们还表明，在抗生素恢复期间补充乳酸杆菌可以逆转抗生素治疗的有害影响。他们的发现提供了一种简单的基于微生物的解决方案，旨在减少蜜蜂疾病负担，外源性物质的环境污染和抗菌素耐药性的传播。
Widespread antibiotic usage in apiculture contributes substantially to the global dissemination of antimicrobial resistance and has the potential to negatively influence bacterial symbionts of honey bees (Apis mellifera). Here, we show that routine antibiotic administration with oxytetracycline selectively increased tetB (efflux pump resistance gene) abundance in the gut microbiota of adult workers while concurrently depleting several key symbionts known to regulate immune function and nutrient metabolism such as Frischella perrera and Lactobacillus Firm-5 strains. These microbial changes were functionally characterized by decreased capped brood counts (marker of hive nutritional status and productivity) and reduced antimicrobial capacity of adult hemolymph (indicator of immune competence). Importantly, combination therapy with three immunostimulatory Lactobacillus strains could mitigate antibiotic-associated microbiota dysbiosis and immune deficits in adult workers, as well as maximize the intended benefit of oxytetracycline by suppressing larval pathogen loads to near-undetectable levels. We conclude that microbial-based therapeutics may offer a simple but effective solution to reduce honey bee disease burden, environmental xenobiotic exposure, and spread of antimicrobial resistance. Daisley et al. show that antibiotic treatment with oxytetracycline impairs the gut microbiota and immune system of honey bees, and reduces capped brood counts. They also show that supplementation with lactobacilli during antibiotic recovery can reverse the harmful effects of the antibiotic treatment. Their findings offer a simple microbial-based solution that aims to reduce honey bee disease burden, environmental pollution by xenobiotics, and spread of antimicrobial resistance.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
11
作者：
Daisley, Brendan A.;Pitek, Andrew P.;Reid, Gregor
通讯作者：
Reid, Gregor
影响因子：
4.6
作者：
Daisley BA;Trinder M;McDowell TW;Welle H;Dube JS;Ali SN;Leong HS;Sumarah MW;Reid G
通讯作者：
Reid G
影响因子：
5.4
作者：
Arredondo, D.;Castelli, L.;Antunez, K.
通讯作者：
Antunez, K.