High-affinity fatty acid-binding activity in epidermis and cultured keratinocytes is attributable to high-molecular-weight and not low-molecular-weight fatty acid-binding proteins.
High-affinity fatty acid-binding activity in epidermis and cultured keratinocytes is attributable to high-molecular-weight and not low-molecular-weight fatty acid-binding proteins.
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表皮和培养的角质形成细胞中的高亲和力脂肪酸结合活性归因于高分子量而不是低分子量脂肪酸结合蛋白。
DOI：
10.1111/1523-1747.ep12354929
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Williams,ML
中科院分区：
文献类型：
--
作者：
Schurer,NY;Bass,NM;Jin,S;Manning,JA;Pillai,S;Williams,ML
脂肪酸结合蛋白（Fatty Acid-binding proteins，FABP）是一种存在于组织中的低分子量蛋白质，参与脂肪酸的代谢。由于表皮也是一个活跃的脂肪生成组织，我们检查了细胞溶胶从小鼠和猪表皮和培养的人角质形成细胞和成纤维细胞的FABP。高亲和力FA结合活性存在于表皮和分化的角质形成细胞中，而在培养的人成纤维细胞或未分化的角质形成细胞中没有发现高亲和力FA结合活性。通过柱层析，在高分子量范围（90-100 kDa）内鉴定出单一结合峰，在低分子量范围（14-15 kDa）内没有明显的结合活性，而在低分子量范围内，常规的FABPs是不稳定的。此外，兔抗大鼠心脏FABP，抗大鼠肠FABP，和抗大鼠肝FABP抗血清没有识别蛋白质在14-15 kDa的范围内在小鼠表皮细胞质中的Western免疫印迹，而抗大鼠心脏抗体识别的蛋白质约32 kDa。分化的角质形成细胞胞质溶胶的等电聚焦显示了具有pI为1.4.0的单个FA结合峰。通过SDS-PAGE分析该结合峰，显示约66和38 kDa的肽。这些研究结果表明，在角质形成细胞胞质溶胶中的FA结合蛋白通常作为异二聚体存在的可能性。用兔抗人血清白蛋白抗体染色的分化的角质形成细胞胞质溶胶和角质形成细胞条件培养基的Western免疫印迹鉴定了约67 kDa的蛋白质，但电聚焦部分不与该抗体反应。因此，表皮和分化的角质形成细胞具有高亲和力的细胞溶质FA结合活性，不能归因于传统的低分子量FABP或白蛋白。
Fatty acid-binding proteins (FABPs) are abundant low-molecular-weight cytosolic proteins in tissues involved in fatty acid (FA) metabolism. Because epidermis is also an active lipogenic tissue, we examined cytosols from murine and porcine epidermis and cultured human keratinocytes and fibroblasts for FABPs. High-affinity FA-binding activity was present in both epidermis and differentiated keratinocytes, whereas no high-affinity FA-binding activity was found in cultured human fibroblasts or undifferentiated keratinocytes. By column chromatography, a single binding peak was identified in the high (90–100 kDa)-molecular-weight range and no binding activity was evident in the low (14–15 kDa)-molecular-weight range, where conventional FABPs elute. Moreover, rabbit anti-rat heart FABP, anti-rat intestine FABP, and anti-rat liver FABP antisera did not identify proteins in the 14–15-kDa range in murine epidermal cytosol by Western immunoblots, whereas the anti-rat-heart antibody recognized a protein of approximately 32 kDa. Isoelectric focusing of differentiated keratinocyte cytosol demonstrated a single FA-binding peak having a pI of ≈ 4.0. Analysis of this binding peak by SDS-PAGE revealed peptides of approximately 66 and 38 kDa. These findings suggest the possibility that the FA-binding protein in keratinocyte cytosol normally exists as a heterodimer. Western immunoblots of both differentiated keratinocyte cytosol and keratinocyte-conditional media stained with a rabbit anti-human serum albumin antibody identified a protein of approximately 67 kDa, but the electrofocused fraction did not react with this antibody. Thus, epidermis and differentiated keratinocytes possess high-affinity cytosolic FA-binding activity that cannot be ascribed either to conventional low-molecular-weight FABPs or to albumin.
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DOI：
10.1016/0006-291x(86)90314-1
发表时间：
1986-06
影响因子：
3.1
作者：
N. Bass;J. Manning
通讯作者：
N. Bass;J. Manning
肾移植受者和孕妇中 HPV 16 E4 和 E7 蛋白的血清反应性。
DOI：
10.1111/1523-1747.ep12499820
发表时间：
1992
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
I. Jochmus;J. Bavinck;F. Claas;A. Schneider;F. J. van der Woude;L. Gissmann
通讯作者：
L. Gissmann
脂肪酸与表皮胞质蛋白的特异性高亲和力结合。
DOI：
10.1111/1523-1747.ep12480586
发表时间：
1991
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Raza,H;Chung,WL;Mukhtar,H
通讯作者：
Mukhtar,H
人表皮和皮脂囊中的细胞视黄酸结合蛋白。
DOI：
10.1111/1523-1747.ep12259164
发表时间：
1984
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
S. M. Puhvel;M. Sakamoto
通讯作者：
M. Sakamoto
培养的人角质形成细胞的脂质含量和代谢的密度依赖性变化。
DOI：
10.1111/1523-1747.ep12463297
发表时间：
1988
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Williams,ML;Rutherford,SL;Ponec,M;Hincenbergs,M;Placzek,DR;Elias,PM
通讯作者：
Elias,PM