Chronic exposure to a glyphosate-containing pesticide leads to mitochondrial dysfunction and increased reactive oxygen species production in Caenorhabditis elegans.
Chronic exposure to a glyphosate-containing pesticide leads to mitochondrial dysfunction and increased reactive oxygen species production in Caenorhabditis elegans.
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DOI：
10.1016/j.etap.2017.11.005
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发表时间：
2018-01
影响因子：
4.3
通讯作者：
Fitsanakis VA
中科院分区：
文献类型：
--
作者：
Bailey DC;Todt CE;Burchfield SL;Pressley AS;Denney RD;Snapp IB;Negga R;Traynor WL;Fitsanakis VA
草甘膦除草剂是世界上使用最广泛的除草剂之一。虽然草甘膦本身相对无毒，但越来越多的证据表明，商业除草剂配方可能会导致氧化应激和线粒体抑制增加。为了评估这些机制在体内，我们长期（24小时）暴露秀丽隐杆线虫不同浓度的草甘膦除草剂触地（TD）。TD暴露后，我们评估了特定的线粒体电子传递链复合物的功能。初始耗氧量研究表明，与对照组相比，中TD浓度和高TD浓度处理组具有抑制作用。从四甲基罗丹明乙酯和ATP测定结果表明，质子梯度和ATP水平的减少，分别。设计了额外的研究以确定TD暴露是否导致活性氧（ROS）产生增加。来自过氧化氢的数据，而不是超氧化物或羟基自由基，分析显示这种特定的ROS在统计学上显著增加。总之，这些数据表明，暴露于TD的秀丽隐杆线虫导致线粒体抑制和过氧化氢的产生。
Glyphosate-containing herbicides are among the most widely-used in the world. Although glyphosate itself is relatively non-toxic, growing evidence suggests that commercial herbicide formulations may lead to increased oxidative stress and mitochondrial inhibition. In order to assess these mechanisms in vivo, we chronically (24 h) exposed Caenorhabditis elegans to various concentrations of the glyphosate-containing herbicide TouchDown (TD). Following TD exposure, we evaluated the function of specific mitochondrial electron transport chain complexes. Initial oxygen consumption studies demonstrated inhibition in mid- and high-TD concentration treatment groups compared to controls. Results from tetramethylrhodamine ethyl ester and ATP assays indicated reductions in the proton gradient and ATP levels, respectively. Additional studies were designed to determine whether TD exposure resulted in increased reactive oxygen species (ROS) production. Data from hydrogen peroxide, but not superoxide or hydroxyl radical, assays showed statistically significant increases in this specific ROS. Taken together, these data indicate that exposure of Caenorhabditis elegans to TD leads to mitochondrial inhibition and hydrogen peroxide production.
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影响因子：
6.1
作者：
Hauser DN;Hastings TG
通讯作者：
Hastings TG
影响因子：
2.9
作者：
Coune, P. G.;Craveiro, M.;Gruetter, R.
通讯作者：
Gruetter, R.
DOI：
10.1080/21624054.2016.1230585
发表时间：
2016-01-01
期刊：
Worm
影响因子：
--
作者：
Detienne, Giel;Van de Walle, Pieter;Temmerman, Liesbet
通讯作者：
Temmerman, Liesbet
影响因子：
5.9
作者：
Benbrook, Charles M.
通讯作者：
Benbrook, Charles M.
影响因子：
7.4
作者：
Leiers, BR;Kampkötter, A;Henkle-Dührsen, K
通讯作者：
Henkle-Dührsen, K